anti-Rat (Rattus) CHEK1 antibody for Immunofluorescence

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CAMK/CAMKL/CHK1 protein kinase Chk1
  • checkpoint kinase 1 S homeolog
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1.S
  • Chek1
  • chek1
Epitope
AA 271-320, pSer301
69
64
38
33
16
15
15
15
14
11
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
477
192
161
14
14
10
10
10
7
5
4
4
4
2
1
1
Host
Rabbit
413
53
23
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
403
176
139
74
66
40
25
21
21
10
7
6
6
5
Options
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Catalog No. ABIN1531555
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.115504 ABIN1532571 IF ELISA Rabbit IgG AA 271-320 Log in to see Polyclonal 1
12.115504 ABIN2705867 IC IF IHC WB Rabbit Center Log in to see Polyclonal
12.115504 ABIN2705868 IC IF IHC WB Rabbit Center Log in to see Polyclonal
12.115504 ABIN2957528 IC IF WB Rabbit pSer301 Log in to see Polyclonal
1 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Log in to see Polyclonal
1 ABIN3183901 ELISA IF IHC WB Rabbit IgG Ser2484 Log in to see Polyclonal
1 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal
1 ABIN1871824 FACS IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN407619 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
1 ABIN3197993 IC IF IHC WB Rabbit pSer345 Log in to see Polyclonal
1 ABIN2888805 IF ELISA Rabbit IgG AA 271-320 Log in to see Polyclonal
1 ABIN265347 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2883630 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal
1 ABIN2594896 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN2594897 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN1575614 IF ELISA Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN2983591 ICC FACS IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN1575611 IF ELISA WB Rabbit IgG pSer301 Log in to see Polyclonal
1 ABIN2857781 ICC IF WB Rabbit pSer301 Log in to see Polyclonal

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope AA 271-320, pSer301
(69), (64), (38), (33), (16), (15), (15), (15), (14), (11), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(477), (192), (161), (14), (14), (10), (10), (10), (7), (5), (4), (4), (4), (2), (1), (1)
Host Rabbit
(413), (53), (23), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(403), (176), (139), (74), (66), (40), (25), (21), (21), (10), (7), (6), (6), (5)
Pubmed 2 references available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN1531555) Images
Specificity

Chk1 (Phospho-Ser301) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser301.

PhosphorylationH:S301 M:S301 R:S301

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser301.
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background

Synonyms: CHEK1, Chk1, kinase Chk1

NCBI Gene Symbol: CHK1

Molecular Weight 54 kDa
Gene ID 1111
OMIM 603078
UniProt O14757
Research Area Cancer, Cell Cycle, DNA/RNA, Kinases/Phosphatases
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Application Notes WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
Comment

Unigene-Number: Hs.24529 (NCBI Gene Symbol: CHK1)

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-CHEK1 antibody (ABIN1531555)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Matsuoka, Ballif, Smogorzewska, McDonald, Hurov, Luo, Bakalarski, Zhao, Solimini, Lerenthal, Shiloh, Gygi, Elledge: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007

Sanchez, Wong, Thoma, Richman, Wu, Piwnica-Worms, Elledge: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN1531555) back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 271-320), (pSer301) antibody (ABIN1531555) Western blot analysis of extracts from 293 cells, using Chk1 (Phospho-Ser301) Antibod...
Immunofluorescence (IF) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 271-320), (pSer301) antibody (ABIN1531555) Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The ...