anti-Human CHEK2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CHEK2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • cds1
  • chek2
  • chk2
  • hucds1
  • lfs2
  • pp1425
  • rad53
  • checkpoint kinase 2
  • serine/threonine-protein kinase chk2
  • checkpoint kinase 2 L homeolog
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • chek2.L
Epitope
AA 111-141, N-Term
53
53
38
34
19
19
19
17
15
15
10
8
7
7
5
5
5
5
5
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
503
228
204
25
18
16
13
8
5
4
3
1
1
1
1
1
Host
Rabbit
387
118
8
1
1
Clonality
Polyclonal
Conjugate
This CHEK2 antibody is un-conjugated
10
10
9
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
418
152
149
103
98
66
50
44
33
10
6
4
2
1
1
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Catalog No. ABIN1881196
$ 385.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19 ABIN1497497 FACS IHC IHC (p) IP WB Mouse IgG2b Log in to see 2H5 0
19 ABIN1497496 FACS IF IHC IHC (p) IP WB Mouse IgG1 Log in to see 5C4 0
19 ABIN1497499 FACS IHC IHC (p) IP WB Mouse IgG1 Log in to see 5C7 0
19 ABIN1497498 FACS IHC IHC (p) IP WB Mouse IgG2b Log in to see 4C10 0
19 ABIN1497495 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 10G7 0
19 ABIN564867 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 1-543 Log in to see 4B7 0
16 ABIN1497500 FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8D11 0
13.252602 ABIN4901263 ELISA IF IHC (p) WB Mouse IgG2b AA 481-531 Log in to see 1C12B8 0
11.5 ABIN685867 IF (p) IHC (p) WB Rabbit IgG AA 120-160 Log in to see Polyclonal 2
11.5 ABIN703165 IF (p) IHC (p) WB Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal 2
11.5 ABIN546186 IHC (p) WB Rabbit Log in to see Polyclonal 3
11.494791 ABIN3043811 IHC (p) WB Rabbit IgG AA 465-498, C-Term Log in to see Polyclonal 0
11.494791 ABIN213892 IHC IHC (p) WB Mouse IgG1 Log in to see 73C175-1-1 0
11.494791 ABIN213921 ICC IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
11.474512 ABIN4297968 FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 Transcript Variant 1 Log in to see 5C4 0
11.474512 ABIN5952373 ICC IF IHC IHC (p) IP WB Rabbit IgG AA 1-200 Log in to see SC604 0
10 ABIN487312 IHC (p) IP WB Mouse IgG2a Log in to see DCS-270 4
10 ABIN487488 IHC (p) IP WB Mouse IgG2a Log in to see DCS-273 0
10 ABIN524632 IHC (p) ELISA WB Mouse IgG2a kappa AA 1-543 Log in to see 3E10 0
10 ABIN524634 IHC (p) ELISA WB Mouse IgG2a kappa AA 1-543 Log in to see 4F10 0

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 111-141, N-Term
(53), (53), (38), (34), (19), (19), (19), (17), (15), (15), (10), (8), (7), (7), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(503), (228), (204), (25), (18), (16), (13), (8), (5), (4), (3), (1), (1), (1), (1), (1)
Host Rabbit
(387), (118), (8), (1), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CHEK2 antibody is un-conjugated
(10), (10), (9), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(418), (152), (149), (103), (98), (66), (50), (44), (33), (10), (6), (4), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-CHEK2 Antibody

Target Details CHEK2 Application Details Handling References for anti-CHEK2 antibody (ABIN1881196) Images
Purification This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Immunogen This CHEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-141 amino acids from the N-terminal region of human CHEK2.
Clone RB19067
Plasmids, Primers & others

Target Details CHEK2

Product Details anti-CHEK2 Antibody Application Details Handling References for anti-CHEK2 antibody (ABIN1881196) Images back to top
Antigen
Alternative Name CHEK2 (CHEK2 Antibody Abstract)
Background CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53.

Synonyms: CHEK2, CDS1, CHK2, RAD53, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2
NCBI Accession NP_009125, NP_665861, NP_001244316, NP_001005735
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK2 Antibody Target Details CHEK2 Handling References for anti-CHEK2 antibody (ABIN1881196) Images back to top
Application Notes WB: 1:1000,IHC (p): 1:50-100,FACS: 1:10-50,IF: 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details References for anti-CHEK2 antibody (ABIN1881196) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

References for anti-CHEK2 antibody (ABIN1881196)

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling Images back to top
Product cited in:

Xiao, Zheng, Huang, Liu, Zhang, Ma: "Deficiency of 53BP1 inhibits the radiosensitivity of colorectal cancer." in: International journal of oncology, Vol. 49, Issue 4, pp. 1600-8, 2017 Method employed by authors: Western Blotting (WB)

Yao, Huang, Zheng, Liu, Lin, Zhang, Yang, Zhang, Ma: "53BP1 loss induces chemoresistance of colorectal cancer cells to 5-fluorouracil by inhibiting the ATM-CHK2-P53 pathway." in: Journal of cancer research and clinical oncology, Vol. 143, Issue 3, pp. 419-431, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Yang, Wood, Hrushesky: "Mammalian TIMELESS is required for ATM-dependent CHK2 activation and G2/M checkpoint control." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3030-4, 2010

Varmark, Kwak, Theurkauf: "A role for Chk2 in DNA damage induced mitotic delays in human colorectal cancer cells." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 2, pp. 312-20, 2010

Zhu, Miao, Huang, Feng, Zhang, Lu, Cai, Tong, Xu, Qian, Ding: "Naphthalimides induce G(2) arrest through the ATM-activated Chk2-executed pathway in HCT116 cells." in: Neoplasia (New York, N.Y.), Vol. 11, Issue 11, pp. 1226-34, 2009

Images

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling References for anti-CHEK2 antibody (ABIN1881196) back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 111-141), (N-Term) antibody (ABIN1881196) CHEK2 Antibody (N-term) (ABIN1881196) flow cytometric analysis of K562 cells (right h...
Flow Cytometry (FACS) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 111-141), (N-Term) antibody (ABIN1881196) CHEK2 Antibody (N-term) (ABIN1881196) flow cytometric analysis of K562 cells (right h...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 111-141), (N-Term) antibody (ABIN1881196) CHEK2 Antibody (N-term) (ABIN1881196) flow cytometric analysis of K562 cells (right h...
Immunofluorescence (IF) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 111-141), (N-Term) antibody (ABIN1881196) CHEK2 Antibody (N-term) (ABIN1881196) flow cytometric analysis of K562 cells (right h...
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 111-141), (N-Term) antibody (ABIN1881196) All lanes : Anti-CHEK2 Antibody (N-term) at 1:1000 dilution Lane 1: HCT116 whole cell...