anti-Human CHEK2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CHEK2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • cds1
  • chek2
  • chk2
  • hucds1
  • lfs2
  • pp1425
  • rad53
  • checkpoint kinase 2
  • serine/threonine-protein kinase chk2
  • checkpoint kinase 2 L homeolog
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • chek2.L
Epitope
pThr68
50
50
40
35
20
17
15
15
15
13
10
9
7
7
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
471
213
193
19
19
16
13
8
5
4
2
1
1
1
1
1
1
Host
Rabbit
345
127
8
1
1
Clonality
Polyclonal
Conjugate
This CHEK2 antibody is un-conjugated
9
9
8
7
7
5
5
5
5
5
5
5
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
387
138
116
104
87
66
43
40
27
6
6
4
2
2
1
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Catalog No. ABIN408359
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.359111 ABIN3043811 IHC (p) WB Rabbit IgG AA 465-498, C-Term Log in to see Polyclonal 0
12.359111 ABIN213892 IHC IHC (p) WB Mouse IgG1 Log in to see 73C175-1-1 0
12.359111 ABIN213921 ICC IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
12.359111 ABIN4297968 FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 Transcript Variant 1 Log in to see 5C4 0
12.359111 ABIN498829 IHC (p) Rabbit pThr387 Log in to see Polyclonal 0
12.359111 ABIN4297966 IHC IHC (p) WB Mouse IgG1 Log in to see 73C175-1-1 0
12.359111 ABIN498830 IHC (p) Rabbit pThr68 Log in to see Polyclonal 0
12.359111 ABIN4297967 ELISA ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG2b Log in to see 1C12B8 0
12.359111 ABIN498918 IHC (p) Rabbit Log in to see Polyclonal 0
12.359111 ABIN498917 IHC (p) Rabbit Log in to see Polyclonal 0
12.359111 ABIN4297972 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
12.359111 ABIN4297973 IHC IHC (p) IP WB Mouse IgG2a Log in to see DCS-270 0
12.359111 ABIN4297977 IHC (p) WB Mouse IgG1 Log in to see 73C175-1-1 0
1 ABIN1497497 FACS IHC IHC (p) IP WB Mouse IgG2b Log in to see 2H5 0
1 ABIN1497496 FACS IF IHC IHC (p) IP WB Mouse IgG1 Log in to see 5C4 0
1 ABIN1497499 FACS IHC IHC (p) IP WB Mouse IgG1 Log in to see 5C7 0
1 ABIN1497498 FACS IHC IHC (p) IP WB Mouse IgG2b Log in to see 4C10 0
1 ABIN1497495 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 10G7 0
1 ABIN1497500 FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8D11 0
1 ABIN302248 IHC (p) WB Mouse IgG1 Log in to see 73C175-1-1 6

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope pThr68
(50), (50), (40), (35), (20), (17), (15), (15), (15), (13), (10), (9), (7), (7), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(471), (213), (193), (19), (19), (16), (13), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(345), (127), (8), (1), (1)
Clonality
Polyclonal
Conjugate This CHEK2 antibody is un-conjugated
(9), (9), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(387), (138), (116), (104), (87), (66), (43), (40), (27), (6), (6), (4), (2), (2), (1)
Pubmed 7 references available
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Product Details anti-CHEK2 Antibody

Target Details CHEK2 Application Details Handling References for anti-CHEK2 antibody (ABIN408359) Images
Specificity Chk2 [p Thr68] Antibody detects endogenous levels of Chk2 protein only when phosphorylated at T68.
Purification Immunogen affinity purified
Immunogen Synthetic phosphopeptide derived from human Chk2 around the phosphorylation site of Threonine 68.

Target Details CHEK2

Product Details anti-CHEK2 Antibody Application Details Handling References for anti-CHEK2 antibody (ABIN408359) Images back to top
Antigen
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background Gene Symbol: CHEK2
Molecular Weight Theoretical MW: 62 kDa
Gene ID 11200
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK2 Antibody Target Details CHEK2 Handling References for anti-CHEK2 antibody (ABIN408359) Images back to top
Application Notes Western Blot 1:500-1:1000, Immunohistochemistry, Immunohistochemistry-Paraffin 1:50-1:200Western blot (WB) analysis of p-Chk2 [p Thr68] pAb in extracts from NIH/3T3 cells treated with UV. Immunohistochemistry (IHC) analyzes of p-Chk2 [p Thr68] pAb in paraffin-embedded human breast carcinoma tissue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details References for anti-CHEK2 antibody (ABIN408359) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.2)
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CHEK2 antibody (ABIN408359)

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling Images back to top
Product cited in:

Ercilla, Llopis, Feu, Aranda, Ernfors, Freire, Agell: "New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress." in: Nucleic acids research, Vol. 44, Issue 10, pp. 4745-62, 2016 Method employed by authors: Immunohistochemistry (IHC)

Domínguez, Feijoo, Bernal, Ercilla, Agell, Genescà, Tusell: "Centrosome aberrations in human mammary epithelial cells driven by cooperative interactions between p16INK4a deficiency and telomere-dependent genotoxic stress." in: Oncotarget, Vol. 6, Issue 29, pp. 28238-56, 2015 (Sample species: Human). Further details: Western Blotting

Rao, Tortola, Perlot, Wirnsberger, Novatchkova, Nitsch, Sykacek, Frank, Schramek, Komnenovic, Sigl, Aumayr, Schmauss, Fellner, Handschuh, Glösmann, Pasierbek, Schlederer, Resch, Ma, Yang, Popper et al.: "A dual role for autophagy in a murine model of lung cancer. ..." in: Nature communications, Vol. 5, pp. 3056, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Ma, Wei, Dong, Huo, Dong: "DNA damage response in renal ischemia-reperfusion and ATP-depletion injury of renal tubular cells." in: Biochimica et biophysica acta, Vol. 1842, Issue 7, pp. 1088-96, 2014 Further details: Western Blotting

Velimezi, Liontos, Vougas, Roumeliotis, Bartkova, Sideridou, Dereli-Oz, Kocylowski, Pateras, Evangelou, Kotsinas, Orsolic, Bursac, Cokaric-Brdovcak, Zoumpourlis, Kletsas, Papafotiou, Klinakis et al.: "Functional interplay between the DNA-damage-response kinase ATM and ARF tumour suppressor protein in human cancer. ..." in: Nature cell biology, Vol. 15, Issue 8, pp. 967-77, 2013 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Evangelou, Bartkova, Kotsinas, Pateras, Liontos, Velimezi, Kosar, Liloglou, Trougakos, Dyrskjot, Andersen, Papaioannou, Drosos, Papafotiou, Hodny, Sosa-Pineda, Wu, Klinakis, Ørntoft, Lukas, Bartek et al.: "The DNA damage checkpoint precedes activation of ARF in response to escalating oncogenic stress during tumorigenesis. ..." in: Cell death and differentiation, Vol. 20, Issue 11, pp. 1485-97, 2013 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Schramek, Kotsinas, Meixner, Wada, Elling, Pospisilik, Neely, Zwick, Sigl, Forni, Serrano, Gorgoulis, Penninger: "The stress kinase MKK7 couples oncogenic stress to p53 stability and tumor suppression." in: Nature genetics, Vol. 43, Issue 3, pp. 212-9, 2011 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling References for anti-CHEK2 antibody (ABIN408359) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody (ABIN408359) Immunohistochemistry-Paraffin: p-Chk2 [p Thr68] Antibody [ABIN4083592] - Paraffin-emb...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody (ABIN408359) Immunohistochemistry-Paraffin: p-Chk2 [p Thr68] Antibody [ABIN4083592] - Human breast...
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody (ABIN408359) Western Blot: p-Chk2 [p Thr68] Antibody [ABIN4083592] - (WB) analysis of p-Chk2 [p Th...
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody (ABIN408359) Western Blot: p-Chk2 [p Thr68] Antibody [ABIN4083592] - Lane1:MCF-7 whole cell lysate...