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|Antigen||Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies|
|Conjugate||This GADD45A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-GADD45A AntibodyTarget Details GADD45A Application Details Handling References for anti-GADD45A antibody (ABIN453659) Images
|Specificity||This antibody reacts to GADD45A.|
|Purification||Saturated Ammonium Sulfate (SAS) precipitation|
|Immunogen||KLH conjugated synthetic peptide selected from the C-terminal region of human GADD45A|
Target Details GADD45AProduct Details anti-GADD45A Antibody Application Details Handling References for anti-GADD45A antibody (ABIN453659) Images back to top
|Alternative Name||GADD45A (GADD45A Antibody Abstract)|
|Background||GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.Synonyms: DDIT-1, DDIT1, DNA damage-inducible transcript 1 protein, GADD45, Growth arrest and DNA damage-inducible protein GADD45 alpha|
|Pathways||p53 Signaling, Cell Division Cycle|
Application DetailsProduct Details anti-GADD45A Antibody Target Details GADD45A Handling References for anti-GADD45A antibody (ABIN453659) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GADD45A Antibody Target Details GADD45A Application Details References for anti-GADD45A antibody (ABIN453659) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-GADD45A antibody (ABIN453659)Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling Images back to top
Li, Morales, Hwang, Wagner, Boothman: "DNA mismatch repair-dependent activation of c-Abl/p73alpha/GADD45alpha-mediated apoptosis." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21394-403, 2008
Zhu, Ren, Korchin, Lahat, Dicker, Lu, Mills, Pollock, Lev: "Soft tissue sarcoma cells are highly sensitive to AKT blockade: a role for p53-independent up-regulation of GADD45 alpha." in: Cancer research, Vol. 68, Issue 8, pp. 2895-903, 2008
Al-Romaih, Sadikovic, Yoshimoto, Wang, Zielenska, Squire: "Decitabine-induced demethylation of 5' CpG island in GADD45A leads to apoptosis in osteosarcoma cells." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 5, pp. 471-80, 2008
Kearsey, Coates, Prescott, Warbrick, Hall: "Gadd45 is a nuclear cell cycle regulated protein which interacts with p21Cip1." in: Oncogene, Vol. 11, Issue 9, pp. 1675-83, 1995
ImagesProduct Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling References for anti-GADD45A antibody (ABIN453659) back to top