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|Antigen||Exocyst Complex Component 4 (EXOC4) Antibodies|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Trout Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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|Specificity||Detects an ~110 kD protein, corresponding to the apparent molecular mass of rSec8 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, chicken, fish (rainbow trout) guinea pig, hamster, monkey, pig, rabbit and sheep origins.|
|Purification||Protein G purified|
Recombinant rat rSec8 (his-tagged) expressed in E. coli.
Type of Immunogen: Recombinant protein
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|Alternative Name||EXOC4 / SEC8 (EXOC4 Antibody Abstract)|
Name/Gene ID: EXOC4
Synonyms: EXOC4, Exocyst complex component Sec8, Exocyst complex component 4, KIAA1699, Sec8p, SEC8-like 1 (S. cerevisiae), SEC8, SEC8-like 1, SEC8L1
|Pathways||Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis|
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Approved: IHC-P (5 μg/mL), IP (12.5 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
|Restrictions||For Research Use only|
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|Buffer||PBS, pH 7.2, 0.1 mM PMSF, 50 % glycerol.|
|Handling Advice||Avoid freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C
Short term: +4°C
Avoid freeze-thaw cycles.
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