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|Antigen||Exocyst Complex Component 4 (EXOC4) Antibodies|
|Epitope||AA 31-201 Alternatives|
|Reactivity||Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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|Cross-Reactivity||Dog (Canine), Mouse (Murine), Chicken, Human|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Rat Sec8 aa. 31-201|
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|Alternative Name||Sec8 (EXOC4 Antibody Abstract)|
|Background||Signal transmission between neurons is regulated by the release of neurotransmitters at the synapse. This process, which is controlled by a complex pathway of membrane trafficking in the presynaptic nerve terminal, leads to membrane fusion and neurotransmitter secretion. Sec8 is a hydrophilic protein of 975 amino acids that is highly expressed in brain and kidney. It is homologus with the yeast secretory protein, Sec8p. In yeast, Sec8 is essential for the Golgi-to-plasma membrane traffic of proteins during constitutive secretion. In rat brain, Sec8 colocalizes with Rab3 and Syntaxin-1a, two important proteins that regulate the fusion of the synaptic vesicle to the plasma membrane. The primary structure of Sec8, a coiled-coil domain (residues 34-99), is commonly found in other proteins important in secretory pathways. This antibody is routinely tested by western blot analysis.|
|Molecular Weight||110 kDa|
|Pathways||Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis|
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Related Products: ABIN968619, ABIN967389
|Restrictions||For Research Use only|
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|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
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|Product cited in:||
Charron, Nakamura, Bacallao, Wandinger-Ness: "Compromised cytoarchitecture and polarized trafficking in autosomal dominant polycystic kidney disease cells." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 111-24, 2000
Duncan, Don-Wauchope, Tapechum, Shipston, Chow, Estibeiro: "High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells." in: The Biochemical journal, Vol. 342 Pt 3, pp. 497-501, 1999
Hazuka, Hsu, Scheller: "Characterization of a cDNA encoding a subunit of the rat brain rsec6/8 complex." in: Gene, Vol. 187, Issue 1, pp. 67-73, 1997
Ting, Hazuka, Hsu, Kirk, Bean, Scheller: "rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 21, pp. 9613-7, 1995
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