Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 118-131), (Internal Region) Peptide
HSPA5
Reactivity: Human
Host: Synthetic
BP, Imm
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- Target See all GRP78 (HSPA5) products
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
- Protein Region
- Internal Region, AA 118-131
- Origin
- Human
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Immunogen (Imm)
- Sequence
- KVVEKKTKPY IQVD
- Predicted Reactivity
- Human
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Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 20-650) peptide (His tag)
HSPA5 Reactivity: Human Host: Escherichia coli (E. coli) SDS
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- Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 75 kDa band observed in lysates of cell lines HepG2, HeLa and MCF7 (calculated MW of 72.3 kDa according to NP_005338.1). Recommended concentration: 0.1-0.3 μg/mL.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
- Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
- Storage
- -20 °C
- Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- Target
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
- Synonyms
- GRP78 Peptide, BiP Peptide, GRP-78 Peptide, grp78 Peptide, hspa5a Peptide, BIP Peptide, MIF2 Peptide, AL022860 Peptide, AU019543 Peptide, Bip Peptide, D2Wsu141e Peptide, D2Wsu17e Peptide, Grp78 Peptide, Hsce70 Peptide, SEZ-7 Peptide, Sez7 Peptide, baffled Peptide, mBiP Peptide, cb865 Peptide, fb60h09 Peptide, fi36d04 Peptide, wu:fb60h09 Peptide, wu:fi36d04 Peptide, zgc:55994 Peptide, zgc:77606 Peptide, 78 kDa glucose-regulated protein Peptide, heat shock protein family A (Hsp70) member 5 Peptide, BiP/GRP78 Peptide, glucose-regulated protein 78 Peptide, putative glucose-regulated protein 78 Peptide, Hsp70 family ATPase KAR2 Peptide, heat shock protein family A (Hsp70) member 5 S homeolog Peptide, heat shock 70 kDa protein 5a Peptide, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) Peptide, heat shock protein 5 Peptide, heat shock protein family A member 5 Peptide, CpipJ_CPIJ003550 Peptide, HSPA5 Peptide, grp78 Peptide, LOC100533358 Peptide, BiP/grp78 Peptide, Tc00.1047053506585.40 Peptide, Tb11.02.5450 Peptide, Tb11.02.5500 Peptide, LMJF_28_1200 Peptide, KAR2 Peptide, LOC100135840 Peptide, hspa5.S Peptide, hspa5 Peptide, Hspa5 Peptide
- Gene ID
- 3309
- NCBI Accession
- NP_005338
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