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|Antigen||Interleukin 4 (IL4) Antibodies|
|Conjugate||This IL-4 antibody is un-conjugated Alternatives|
Blocking Antibody (Inhibition), Western Blotting (WB)
|5 references available|
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Product Details anti-IL-4 AntibodyTarget Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN967463) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Purified Recombinant Human IL-4|
Target Details IL-4Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 antibody (ABIN967463) Images back to top
|Alternative Name||IL-4 (IL4 Antibody Abstract)|
|Background||The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described. This antibody is routinely tested as a blocking control for intracellular staining.|
|Pathways||JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation|
Application DetailsProduct Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 antibody (ABIN967463) Images back to top
1. Blocking Control for Intracellular Staining:
The unlabeled MP4-25D2 antibody can be used as a blocking control to demonstrate specificity of IL-4 staining by conjugated MP4-25D2 antibody. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1-10 µg of unlabeled MP4-25D2 antibody (ABIN967463) for 20 minutes at 4°C, prior to staining with conjugated MP4-25D2 antibody. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
The NA/LE™ MP4-25D2 antibody is useful for neutralization of human IL-4 bioactivity. A suitable NA/LE ™ rat IgG1 isotype control to match the MP4-25D2 antibody is the R3-34 antibody.
3. WB: The purified MP4-25D2 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 antibody (ABIN967463) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4° C.|
References for anti-IL-4 antibody (ABIN967463)Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
|Product cited in:||
Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996
Jung, Schauer, Rieger, Wagner, Einsle, Neumann, Heusser: "Interleukin-4 and interleukin-5 are rarely co-expressed by human T cells." in: European journal of immunology, Vol. 25, Issue 8, pp. 2413-6, 1995
Ramanathan, Ingram, Sullivan, Greenberg, Reim, Trotta, Le: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, 1993
Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992
Chrétien, Van Kimmenade, Pearce, Banchereau, Abrams: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, 1989
ImagesProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN967463) back to top