NGFB Protein (AA 122-241)

Catalog No. ABIN1096128

This Recombinant NGFB protein is produced in Escherichia coli (E. coli).

Quick Overview for NGFB Protein (AA 122-241) (ABIN1096128)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 122-241

Purpose: Recombinant Human β-Nerve Growth Factor/β-NGF (Ser122-Ala241, E. coli)

Sequence: SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA

Characteristics: Recombinant Human NGF Beta (beta-Nerve Growth Factor/beta-NGF) produced in E. coli is a non-glycosylated non-covalent homodimer of two 118 amino acid polypeptides each with a molecular mass of 13.4 kDa.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Comment:

Biological activity: ED50 is less than 1.0 ng/ml as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.Specific Activity is greater than 1 x 106 IU/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: NGFB (Nerve Growth Factor beta (NGFB))

Alternative Name: beta-Nerve Growth Factor

Background: Recombinant Human NGF Beta: beta-Nerve Growth Factor/beta-NGF was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits alpha, beta, and gamma, it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Beta-NGF is a neurotrophic factor that signals through its receptor beta-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Beta-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that beta-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Recombinant Human NGF Beta shares 90% sequence similarity with mouse protein and shows cross-species reactivity.ReferencesForsell P, et al. The use of TrkA-PathHunter assay in high-throughput screening to identify compounds that affect nerve growth factor signaling.PMID: 23458757http://www.ncbi.nlm.nih.gov/pubmed/23458757
Alternative Names: Recombinant Human NGF Beta, Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB

Molecular Weight: 13.4 kDa

UniProt: P01138

Pathways: NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size