NGFB Protein

Catalog No. ABIN7320297

This Recombinant NGFB protein is produced in CHO Cells.

Quick Overview for NGFB Protein (ABIN7320297)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: CHO Cells

Application: Cell Culture (CC)

Purity: > 96 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Mouse β-NGF/Beta-NGF Protein

Sequence: Ser 122-Gly 241

Characteristics: A DNA sequence encoding the mature form of mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed, with an initial Met at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 1-8ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM NaAc, 150 mM NaCl, pH 5.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: NGFB (Nerve Growth Factor beta (NGFB))

Alternative Name: beta-NGF/Beta-NGF

Background: Ngfb,Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

Molecular Weight:

Calculated MW: 13.5 kDa

Observed MW: 13.5 kDa

Gene ID: 18049

NCBI Accession: NP_001106168

UniProt: P01139

Pathways: NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size