HBZ Protein (AA 1-142) (His tag)
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- Target See all HBZ Proteins
- HBZ (Hemoglobin, zeta (HBZ))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-142
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HBZ protein is labelled with His tag.
- Purpose
- Recombinant Human Hemoglobin Subunit ζ/HBAZ (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MSLTKTERTI IVSMWAKIST QADTIGTETL ERLFLSHPQT KTYFPHFDLH PGSAQLRAHG SKVVAAVGDA VKSIDDIGGA LSKLSELHAY ILRVDPVNFK LLSHCLLVTL AARFPADFTA EAHAAWDKFL SVVSSVLTEK YR
- Characteristics
- Recombinant Human Hemoglobin Subunit Zeta/HBAZ is produced by our E. coli expression system. The target protein is expressed with sequence (Ser2-Arg142) of Human HBZ fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
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Hemoglobin, zeta (HBZ) (AA 1-142) protein (His tag)
HBZ Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Hemoglobin, zeta (HBZ) (AA 1-142) protein (GST tag)HBZ Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Hemoglobin, zeta (HBZ) (AA 2-142) protein (His tag)Crystallography grade HBZ Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Hemoglobin, zeta (HBZ) (AA 2-142) protein (His tag)Crystallography grade HBZ Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Hemoglobin, zeta (HBZ) protein (Myc-DYKDDDDK Tag)HBZ Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- HBZ (Hemoglobin, zeta (HBZ))
- Alternative Name
- Hemoglobin Subunit zeta/hbaz (HBZ Products)
- Synonyms
- RGD1307486 Protein, HBZ Protein, hba-l1 Protein, MGC82702 Protein, RA_M008_JSM295ECF Protein, hemoglobin, zeta Protein, hemoglobin subunit zeta Protein, HBZ Protein, Hbz Protein, HBZ1 Protein, hbz Protein
- Sub Type
- Fusionprotein
- Background
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Hemoglobin Subunit Zeta (HBZ) is a member of the Globin family. The zeta chain is an alpha-type chain of mammalian embryonic Hemoglobin that is synthesized primarily in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal growth and adult life. The HBZ gene consists of five functional genes and two pseudogenes, the order of genes is 5-zeta-pseudozeta-mu-pseudoalpha-1-alpha-2-alpha-1-theta-1-3.
Alternative Names: Hemoglobin Subunit Zeta, HBAZ, Hemoglobin Zeta Chain, Zeta-Globin, HBZ, HBZ2 - Molecular Weight
- 17.8kDa
- UniProt
- P02008
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