LDHB Protein (AA 2-334) (His tag)

Catalog No. ABIN1097068

Product Details

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Protein Type: Recombinant

Protein Characteristics: AA 2-334

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This LDHB protein is labelled with His tag.

Purpose: Recombinant Human L-Lactate Dehydrogenase B Chain/LDH-B (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL

Characteristics: Recombinant Human L-Lactate Dehydrogenase B Chain/LDH-B is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Leu334) of Human LDHB fused with a His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: LDH-B (LDHB Products)

Synonyms: LDH-B Protein, LDH-H Protein, LDHBD Protein, TRG-5 Protein, AI790582 Protein, H-Ldh Protein, Ldh-2 Protein, Ldh2 Protein, ldh-h Protein, ldh2 Protein, ldhb Protein, ldhba Protein, trg-5 Protein, LDHB Protein, LDH-B-A Protein, wu:fa16d07 Protein, wu:fa20c10 Protein, wu:fa96a08 Protein, LDH-B-B Protein, zgc:92882 Protein, lactate dehydrogenase B Protein, lactate dehydrogenase B S homeolog Protein, lactate dehydrogenase Ba Protein, lactate dehydrogenase Bb Protein, LDHB Protein, Ldhb Protein, ldhb.S Protein, ldhb Protein, ldhba Protein, ldhbb Protein

Sub Type: Fusionprotein

Background: L-Lactate Dehydrogenase B Chain (LDH-B) is a member of the lactate dehydrogenase family that consists of three members, LDH-A, LDH-B and LDH-C, members of this family function as powerful markers for germ cell tumors. LDH-B is an oxidoreductase that catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. It converts pyruvate to lactate when oxygen is absent or in short supply and it performs the reverse reaction during the Cori cycle in the liver. It is also called Hydroxybutyrate Dehydrogenase (HBD) due to its ability to catalyze the oxidation of hydroxybutyrate.
Alternative Names: L-lactate Dehydrogenase B Chain, LDH-B, LDH Heart Subunit, LDH-H, Renal Carcinoma Antigen NY-REN-46, LDHB

Molecular Weight: 38.8 kDa

UniProt: P07195

Pathways: Warburg Effect