p21 Protein (AA 2-164) (His tag)
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- Target See all p21 (CDKN1A) Proteins
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-164
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This p21 protein is labelled with His tag.
- Purpose
- Recombinant Human Cyclin-Dependent Kinase Inhibitor 1/CDKN1A/p21 (C-6His)
- Sequence
- SEPAGDVRQN PCGSKACRRL FGPVDSEQLR RDCDALMAGC IQEARERWNF DFVTETPLEG DFAWERVRGL GLPKLYLPTG PRRGRDELGG GRRPGTSPAL LQGTAEEDHV DLSLSCTLVP RSGEQAEGSP GGPGDSQGRK RRQTSMTDFY HSKRRLIFSK RKPLEHHHHH H
- Characteristics
- Recombinant Human Cyclin-Dependent Kinase Inhibitor 1/CDKN1A is produced by our E. coli expression system. The target protein is expressed with sequence (Ser2-Pro164) of Human CDKN1A fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CDKN1A Protein
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Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 2-164) protein (His-IF2DI Tag)
CDKN1A Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-164) protein (GST tag)CDKN1A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CDKN1A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 2-164) protein (GST tag)CDKN1A Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
- Alternative Name
- p21 (CDKN1A Products)
- Sub Type
- Fusionprotein
- Background
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Cyclin-Dependent Kinase Inhibitor 1 (CDKN1A) is a member of the CDI family. CDKN1A is widely expressed in all adult tissues, but low expressed in the brain tissue. CDKN1A can be induced by p53/TP53, mezerein and IFNB1, repressed by HDAC1. CDKN1A may be an important intermediate, by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage, CDKN1A can bind to and inhibit cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.
Alternative Names: Cyclin-Dependent Kinase Inhibitor 1, CDK-Interacting Protein 1, Melanoma Differentiation-Associated Protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1 - Molecular Weight
- 19.25 kDa
- UniProt
- P38936
- Pathways
- p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
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