Peroxiredoxin 4 Protein (PRDX4) (AA 38-271) (His tag)

Catalog No. ABIN1097320

Product Details

Target: Peroxiredoxin 4 (PRDX4)

Protein Type: Recombinant

Protein Characteristics: AA 38-271

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Peroxiredoxin 4 protein is labelled with His tag.

Purpose: Recombinant Human Peroxiredoxin-4/PRDX4 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN

Characteristics: Recombinant Human Peroxiredoxin-4/PRDX4 is produced by our E. coli expression system. The target protein is expressed with sequence (Trp38-Asn271) of Human PRDX4 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.2.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Peroxiredoxin 4 (PRDX4)

Alternative Name: Peroxiredoxin-4/PRDX4 (PRDX4 Products)

Synonyms: MGC82793 Protein, prdx4 Protein, MGC79732 Protein, PRDX4 Protein, AOE37-2 Protein, PRX-4 Protein, AOE372 Protein, Prx-iv Protein, Prx4 Protein, TRANK Protein, fb59c09 Protein, wu:fb59c09 Protein, zgc:162938 Protein, peroxiredoxin 4 Protein, peroxiredoxin 4 L homeolog Protein, PRDX4 Protein, prdx4.L Protein, prdx4 Protein, Prdx4 Protein

Sub Type: Fusionprotein

Background: Peroxiredoxin-4 (PRDX4) is a member of the AhpC/TSA family. PRDX4 is a cytoplasmic protein and contains one thioredoxin domain. PRDX4 exists in homodimer or heterodimer with PRDX1. PRDX4 reduces hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. In addition, PRDX4 is probably involved in redox regulation of the cell, regulating the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
Alternative Names: Peroxiredoxin-4, Antioxidant Enzyme AOE372, AOE37-2, Peroxiredoxin IV, Prx-IV, Thioredoxin Peroxidase AO372, Thioredoxin-Dependent Peroxide Reductase A0372, PRDX4

Molecular Weight: 33.8 kDa

UniProt: Q13162