SEMA3C Protein (AA 21-738) (His tag)

Catalog No. ABIN1097493

Product Details

Target: SEMA3C (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3C (SEMA3C))

Protein Type: Recombinant

Protein Characteristics: AA 21-738

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This SEMA3C protein is labelled with His tag.

Purpose: Recombinant Human Semaphorin 3C/SEMA3C (C-6His)

Sequence: GSSQPQARVY LTFDELRETK TSEYFSLSHH PLDYRILLMD EDQDRIYVGS KDHILSLNIN NISQEALSVF WPASTIKVEE CKMAGKDPTH GCGNFVRVIQ TFNRTHLYVC GSGAFSPVCT YLNRGRRSED QVFMIDSKCE SGKGRCSFNP NVNTVSVMIN EELFSGMYID FMGTDAAIFR SLTKRNAVRT DQHNSKWLSE PMFVDAHVIP DGTDPNDAKV YFFFKEKLTD NNRSTKQIHS MIARICPNDT GGLRSLVNKW TTFLKARLVC SVTDEDGPET HFDELEDVFL LETDNPRTTL VYGIFTTSSS VFKGSAVCVY HLSDIQTVFN GPFAHKEGPN HQLISYQGRI PYPRPGTCPG GAFTPNMRTT KEFPDDVVTF IRNHPLMYNS IYPIHKRPLI VRIGTDYKYT KIAVDRVNAA DGRYHVLFLG TDRGTVQKVV VLPTNNSVSG ELILEELEVF KNHAPITTMK ISSKKQQLYV SSNEGVSQVS LHRCHIYGTA CADCCLARDP YCAWDGHSCS RFYPTGKRRS RRQDVRHGNP LTQCRGFNLK AYRNAAEIVQ YGVKNNTTFL ECAPKSPQAS IKWLLQKDKD RRKEVKLNER IIATSQGLLI RSVQGSDQGL YHCIATENSF KQTIAKINFK VLDSEMVAVV TDKWSPWTWA SSVRALPFHP KDIMGAFSHS EMQMINQYCK DTRQQHQQGD ESQKMRGDYG KLKALINSVD HHHHHH

Characteristics: Recombinant Human Semaphorin 3C is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Gly21-Ser738) of Human SEMA3C fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: SEMA3C (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3C (SEMA3C))

Alternative Name: semaphorin-3c (SEMA3C Products)

Synonyms: SEMAE Protein, SemE Protein, 1110036B02Rik Protein, Semae Protein, semaphorin 3C Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C Protein, SEMA3C Protein, Sema3c Protein

Sub Type: Fusionprotein

Background: Semaphorin 3C is one of six Class 3 secreted semaphorins, which are potent chemorepellents, that play a role in axon and/or vascular guidance during development can be up-regulated in the process of tumor progression. Semaphorin 3C consists of a 20 amino acids signal sequence, a N-terminal Sema domain, a cysteine knot, a furine-type cleavage site, an Ig-like domain, and a C-terminal basic domain. Semaphorin 3C expressed highly in the heart, colon, skeletal muscle, small intestine, testis, ovary and prostate, expressed lowly in the other organs, including the brain.
Alternative Names: Semaphorin-3C, Semaphorin-E, Sema E, SEMA3C, SEMAE

Molecular Weight: 82.44 kDa

UniProt: Q99985