VEGF Protein (AA 27-191, Isoform 165)

Catalog No. ABIN1097784

This Recombinant VEGF protein is expressed in Human Cells.

Quick Overview for VEGF Protein (AA 27-191, Isoform 165) (ABIN1097784)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 27-191, Isoform 165

Purpose: Recombinant Human VEGF-A/VEGF165

Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Characteristics: Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 weeks

Target Details

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: Vegf

Background: Alternative Names: Vascular Endothelial Growth Factor Isoform 165, VEGF165Recombinant Human VEGF 165: Human Vascular endothelial growth factor, also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
Alternative Names: Recombinant Human VEGF 165, Vascular Endothelial Growth Factor Isoform 165, VEGF165

Molecular Weight: 19.1 kDa

UniProt: P15692-4