MMP8 Protein (Catalytic Domain)
-
- Target See all MMP8 Proteins
- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Catalytic Domain
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Sequence
- NPKWER TNLTYRIRNY TPQLSEAEVE RAIKDAFELW SVASPLIFTR ISQGEADINI AFYQRDHGDN SPFDGPNGIL AHAFQPGQGI GGDAHFDAEE TWTNTSANYN LFLVAAHEFG HSLGLAHSSD PGALMYPNYA FRETSNYSLP QDDIDGIQAI YG
- Characteristics
- Recombinant matrix metalloproteinase-8 (MMP-8, collagenase-2, neurophil collagenase) cloned from human cDNA, expressed in E.coli. The enzyme consists of the catalytic domain of human MMP-8 (residues 105-262)
- Purification
- Folding state checked by NMR.
- Purity
- > 95% by SDS-PAGE.
- Biological Activity Comment
- > 80U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.
- Top Product
- Discover our top product MMP8 Protein
-
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-467) protein (His tag)
MMP8 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-467) protein (His tag)MMP8 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-465) protein (His tag)MMP8 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 1-467) protein (GST tag)MMP8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) proteinMMP8 Origin: Mouse Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE
-
- Application Notes
- Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors
- Comment
-
The enzyme was observed as a single band migrating at a molecular weight of < 20kDa
- Restrictions
- For Research Use only
-
- Buffer
- 0.2mg/ml in Tris 20mM pH 7.2, CaCl2 10mM, ZnCl2 0.1mM, NaCl 0.3M, Acetohydroxamic Acid (AHA) 0.5M.
- Storage
- -20 °C/-80 °C
- Storage Comment
- -80°C. The enzyme is stable at -20°C for at least 1 week. After initial defrost, aliquot enzyme into individual tubes and refreeze at -80°C. Avoid repeated freeze/defrost cycles.
-
- Target
- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
- Alternative Name
- MMP-8 (MMP8 Products)
- Synonyms
- MMP8 Protein, BB138268 Protein, CLG1 Protein, HNC Protein, MMP-8 Protein, PMNL-CL Protein, matrix metallopeptidase 8 Protein, neutrophil collagenase Protein, MMP8 Protein, Mmp8 Protein, LOC100339790 Protein
- Molecular Weight
- 17.7kDa
- UniProt
- P22894
-
