POLR3F Protein (His tag)

Catalog No. ABIN1098506

Product Details

Target: POLR3F (Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This POLR3F protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Purification: purified by chromatography

Purity: > 90 % by SDS - PAGE

Application Details

Comment:

Synonyms: DNA-directed RNA polymerase III subunit RPC6, RPC39, RPC6

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 30% glycerol, 1 MM DTT

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: POLR3F (Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F))

Alternative Name: POLR3F (POLR3F Products)

Synonyms: 2810411G20Rik Protein, 3010019O03Rik Protein, 3110032A07Rik Protein, RPC39 Protein, RPC6 Protein, zgc:56299 Protein, zgc:76983 Protein, RNA polymerase III subunit F Protein, polymerase (RNA) III (DNA directed) polypeptide F Protein, POLR3F Protein, Polr3f Protein, polr3f Protein

Background: POLR3F, also known as RPC39 and RPC6, belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family. POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). It is DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR3F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 38.1kDa (339aa), confirmed by MALDI-TOF

NCBI Accession: NP_006457