BUB3 Protein (AA 1-328) (His tag)
Quick Overview for BUB3 Protein (AA 1-328) (His tag) (ABIN1098754)
Target
See all BUB3 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-328
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Purification tag / Conjugate
- This BUB3 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMTGSNEF KLNQPPEDGI SSVKFSPNTS QFLLVSSWDT SVRLYDVPAN SMRLKYQHTG AVLDCAFYDP THAWSGGLDH QLKMHDLNTD QENLVGTHDA PIRCVEYCPE VNVMVTGSWD QTVKLWDPRT PCNAGTFSQP EKVYTLSVSG DRLIVGTAGR RVLVWDLRNM GYVQQRRESS LKYQTRCIRA FPNKQGYVLS SIEGRVAVEY LDPSPEVQKK KYAFKCHRLK ENNIEQIYPV NAISFHNIHN TFATGGSDGF VNIWDPFNKK RLCQFHRYPT SIASLAFSND GTTLAIASSY MYEMDDTEHP EDGIFIRQVT DAETKPKSPC T
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Purification
- > 85% by SDS-PAGE
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Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-326) protein (His tag)
custom-made BUB3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-328) protein (His tag)custom-made BUB3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-328) protein (GST tag)BUB3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) proteinBUB3 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)BUB3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Synonyms: Mitotic checkpoint protein BUB3, BUB3L, hBUB3
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
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Alternative Name
- Bub3
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Background
- Mitotic checkpoint protein BuB3, also known as BuB3, is a conserved component of the mitotic spindle assembly complex (MCC). The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BuB3 protein. BuB3 is essential for the kinetochore localization of BuB1 and BuBR1. BuB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. In addition, BuB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. Recombinant human BuB3 protein, fused to His-tag at N-terminus, was expressed in E. coli.
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Molecular Weight
- 39.5 kDa (351aa)
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NCBI Accession
- NP_004716
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Pathways
- Maintenance of Protein Location
Target
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