CD31 Protein (His tag)
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- Target See all CD31 (PECAM1) Proteins
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD31 protein is labelled with His tag.
- Application
- Biochemical Assay (BCA)
- Characteristics
- Optimized DNA sequence encoding Human extracellular domain of human CD31including a C-terminal 6His tag was expressed in HEK293 cells.
- Purity
- > 95%, as determined by SDS-PAGE and HPLC
- Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
- Biological Activity Comment
- Recombinant Human CD31 activity was determined by its ability to support the adhesion of Jurkat human leukemic T cells. When cells are added to recombinant human CD31 coated plates ( 3.3 μg/mL , 100 μL/well ) in the presence of PMA , approximately 50% - 65% will adhere specifically after 30 minutes at 37°C
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- Discover our top product PECAM1 Protein
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Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-601) protein (His tag)
PECAM1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 18-590) protein (His tag)PECAM1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-602) protein (His tag)PECAM1 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-601) protein (PE,Fc Tag)PECAM1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-150) protein (His tag)PECAM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 29-738) protein (GST tag)PECAM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 1-738) protein (GST tag)PECAM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Platelet/endothelial Cell Adhesion Molecule (PECAM1) proteinPECAM1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 621-738) protein (His tag)Crystallography grade PECAM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Buffer
- Recombinant CD31 is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
- Storage
- 4 °C/-20 °C
- Storage Comment
- Recombinant CD31, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
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- Target
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
- Alternative Name
- CD31 (PECAM1) (PECAM1 Products)
- Synonyms
- CD31 Protein, CD31/EndoCAM Protein, GPIIA' Protein, PECA1 Protein, PECAM-1 Protein, endoCAM Protein, C85791 Protein, Cd31 Protein, Pecam Protein, platelet/endothelial cell adhesion molecule 1 Protein, platelet and endothelial cell adhesion molecule 1 Protein, pecam1 Protein, PECAM1 Protein, Pecam1 Protein
- Molecular Weight
- 100 kDa
- Pathways
- Regulation of Actin Filament Polymerization
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