IL-10 Protein

Catalog No. ABIN1305072

Product Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Brand: BD Pharmingen™

Specificity: Bioassay: The activity range is measured in a proliferation assay using MC/9 indicator cells. Cells were co-stimulated with recombinant mouse IL-4 (Cat. No. 550067) at 50 pg/ml and were incubated for 24 hours prior to being pulsed. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Specific Activity: 0.5 - 3.3 x 10^7 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation).
ED50: 0.3 - 2.0 ng/ml
Observed dose response: >250 fold

Characteristics: Recombinant human IL-10 is supplied as a frozen, sterile-filtered aqueous buffered solution containing glycerol and biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant human IL-10 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography
Recombinant human IL-10 (Cat No. 554611) is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing glycerol and bovine serum albumin, with no preservatives. Recombinant human IL-10 is ≥ 95 % pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per μg of human IL-10, as measured in a chromogenic LAL assay.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of hIL-10, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant human IL-10 is useful as a quantitative standard for measuring human IL-10 protein levels in an IL-10 specific sandwich ELISA with the purified JES3-9D7 antibody (Cat. No. 554705) as a capture antibody and the biotinylated JES3-12G8 antibody (Cat. 554611) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human IL-10 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring human IL-10 in serum or plasma the BD OptEIA™ Human IL-10 ELISA Set (Cat. No. 555157) and the BD OptEIA™ Human IL-10 ELISA Kit (Cat. No. 550613) are specially formulated and recommended.
Ligand Blocking Control for Immunofluorescent Staining of Cytokines: Recombinant human IL-10 can be used as a blocking control to demonstrate the specificity of IL-10 staining by our conjugated anti-IL-10 antibodies (for example Cat. No. 554498, Cat. No. 554706, Cat. No. 554707).

Comment:

BD Pharmingen™ Recombinant Human IL-10 - Reactivity Hu

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Handling Advice: Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.5 kD protein that shares over 80 % sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of proinflammatory T cell mediated immunity.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Howard, OGarra, Ishida, de Waal Malefyt, de Vries: "Biological properties of interleukin 10." in: Journal of clinical immunology, Vol. 12, Issue 4, pp. 239-47, (1992) (PubMed).

Vieira, de Waal-Malefyt, Dang, Johnson, Kastelein, Fiorentino, deVries, Roncarolo, Mosmann, Moore: "Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 4, pp. 1172-6, (1991) (PubMed).

Target Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10 (IL10 Products)

Synonyms: CSIF Protein, GVHDS Protein, IL-10 Protein, IL10A Protein, TGIF Protein, Il-10 Protein, IL10X Protein, interleukin 10 Protein, IL10 Protein, Il10 Protein

Background: Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.5 kD protein that shares over 80 % sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of proinflammatory T cell mediated immunity.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints