IL-10 Protein

Catalog No. ABIN1305077

Product Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Brand: BD Pharmingen™

Characteristics: The rat IL-10 was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beer-Lambert Law. Recombinant rat IL-10 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered Dulbecco's PBS pH 7.2 and 1.0 mg/ml BSA carrier protein.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of IL-10.

Application Details

Application Notes: ELISA Standard: The recombinant rat IL-10 (Cat. No. 555113) is useful as a quantitative standard for a sandwich ELISA for measuring rat IL-10 protein levels. Purified A5-7 antibody (Cat. No. 555083) is recommended to use as the capture antibody and can be paired with biotinylated A5-6 antibody (Cat. No. 555084), with recombinant rat IL-10 as the standard. To obtain linear standard curves, doubling dilutions of rat IL-10 ranging from ~2,000 to 5 pg/ml are recommended for inclusion in each ELISA plate.
Immunofluorescent Staining and Flow Cytometric Analysis: The recombinant rat IL-10 can be used as a blocking control to demonstrate specificity of IL-10 staining by the PE-A5-4 antibody, (Cat. No. 555088). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity. Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.

Comment:

BD Pharmingen™ Recombinant Rat IL-10

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: 100 μg/mL Frozen aqueous buffered solution containing BSA.

Preservative: Azide free

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less that 0.5 - 1.0 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

Storage: -80 °C

Storage Comment: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less that 0.5 - 1.0 mg/ml carrier protein* such as human or bovine serum albumin, aliquoted and stored at -80°C. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Feng, Tang, Chang, Wilson: "Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages." in: Biochemical and biophysical research communications, Vol. 192, Issue 2, pp. 452-8, (1993) (PubMed).

Fiorentino, Bond, Mosmann: "Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones." in: The Journal of experimental medicine, Vol. 170, Issue 6, pp. 2081-95, (1990) (PubMed).

Target Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10 (IL10 Products)

Synonyms: CSIF Protein, GVHDS Protein, IL-10 Protein, IL10A Protein, TGIF Protein, Il-10 Protein, IL10X Protein, interleukin 10 Protein, IL10 Protein, Il10 Protein

Background: Interleukin-10 (IL-10), (aka Cytokine Synthesis Inhibitory Factor CSIF), is an 18.5 kD protein that shares over 80 % sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of proinflammatory T cell mediated immunity.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints