EPO Protein

Catalog No. ABIN1305139

Recombinant EPO protein expressed in Escherichia coli (E. coli) and has been mentioned in 6+ publications.

Quick Overview for EPO Protein (ABIN1305139)

Target: EPO (Erythropoietin (EPO))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: ≥ 95 %

Product Details

Brand: BD Pharmingen™

Specificity: Biological Activity Measured using TF-1 indicator cells
Specific Activity: 0.1 - 1.0 x 10^8 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation).
ED50: 0.1 - 1.0 ng/ml
Observed linear dose response range: >100 fold

Characteristics: Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, and containing 1 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant mouse EPO is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law.

Purification: The recombinant mouse EPO is > 95 % pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law.

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of mouse EPO, as measured in a chromogenic LAL assay.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD Pharmingen™ Mouse Erythropoietin Recombinant Protein - Reactivity Ms

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, and containing 1 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.

Preservative: Azide free

Handling Advice: Rapidly thaw and quick-spin product prior to use. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

References

Nagao, Suga, Okano, Masuda, Narita, Ikura, Sasaki: "Nucleotide sequence of rat erythropoietin." in: Biochimica et biophysica acta, Vol. 1171, Issue 1, pp. 99-102, (1992) (PubMed).

Krantz: "Erythropoietin." in: Blood, Vol. 77, Issue 3, pp. 419-34, (1991) (PubMed).

Kitamura, Tange, Terasawa, Chiba, Kuwaki, Miyagawa, Piao, Miyazono, Urabe, Takaku: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, (1989) (PubMed).

McDonald, Lin, Goldwasser: "Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene." in: Molecular and cellular biology, Vol. 6, Issue 3, pp. 842-8, (1986) (PubMed).

Shoemaker, Mitsock: "Murine erythropoietin gene: cloning, expression, and human gene homology." in: Molecular and cellular biology, Vol. 6, Issue 3, pp. 849-58, (1986) (PubMed).

JACOBSON, GOLDWASSER, FRIED, PLZAK: "Role of the kidney in erythropoiesis." in: Nature, Vol. 179, Issue 4560, pp. 633-4, (1957) (PubMed).

Target Details

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO

Target Type: Hormone

Background: Mouse erythropoietin (EPO) is a 30 kD heavily glycosylated protein containing 166 amino acids. The carbohydrate residues compose approximately 30 % of the molecule by weight.3 It shares 80 % and 95 % homology with human and rat EPO, respectively. EPO functions as the survival and proliferation factors of late erythroid progenitor cells (CFU-E). In adult mammals, EPO is synthesized almost exclusively in the kidneys.

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity