EPO Protein (His tag)
-
- Target See all EPO Proteins
- EPO (Erythropoietin (EPO))
- Protein Type
- Recombinant
- Biological Activity
- Active
-
Origin
- Mouse
-
Source
- Human Cells
- Purification tag / Conjugate
- This EPO protein is labelled with His tag.
- Purpose
- Recombinant Mouse Erythropoietin/EPO (C-6His)
- Sequence
- Ala27-Arg192
- Characteristics
- Recombinant Mouse Erythropoietin is produced by our Mammalian expression system and the target gene encoding Ala27-Arg192 is expressed with a 6His tag at the C-terminus.
- Purity
- >95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.35 ng/mL.
- Top Product
- Discover our top product EPO Protein
-
Erythropoietin (EPO) (AA 28-193) (Active) protein
EPO Origin: Human Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Erythropoietin (EPO) (AA 1-193) protein (GST tag)EPO Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Erythropoietin (EPO) (AA 27-192) protein (His tag)Crystallography grade EPO Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Target
- EPO (Erythropoietin (EPO))
- Alternative Name
- Erythropoietin/EPO (EPO Products)
- Synonyms
- EPO Protein, EP Protein, MVCD2 Protein, erythropoietin Protein, erythropoietin S homeolog Protein, erythropoietin a Protein, EPO Protein, epo Protein, epo.S Protein, Epo Protein, epoa Protein
- Target Type
- Hormone
- Background
-
Background: Erythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. Erythropoietin is a member of the EPO/TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. The differentiation of CFU-E (Colony Forming Unit-Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy.
Synonym: Erythropoietin, Epoetin, EPO
- Molecular Weight
- 19.4 kDa
- UniProt
- Q0VED9
- Pathways
- JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
-
