Erythropoietin (EPO) (AA 28-193) (Active) Protein

Details for Product No. ABIN987828, Supplier: Log in to see
Protein Name
  • EPO
  • EP
  • MVCD2
  • erythropoietin
  • erythropoietin S homeolog
  • erythropoietin a
  • EPO
  • epo
  • epo.S
  • Epo
  • epoa
Protein Characteristics
AA 28-193
11
10
6
4
4
4
2
2
1
1
1
1
1
1
1
1
Origin
Human
32
14
7
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Source
CHO Cells
21
19
13
9
6
4
2
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
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Characteristics ED50< 1 ng/mL, measured in a cell proliferation assay using TF-1 human erythroleukemic cells, corresponding to a specific activity of >1 x 10^6 units/mg
Purity > 95 % as analyzed by SDS-PAGE and HPLC.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Protein Name
Target Type Hormone
Background Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The production of EPO by kidney cells is increased in response to hypoxia or anemia. Recombinant EPO has been approved for the treatment of anemia associated with chronic renal failure as well as for anemia of AZT treated AIDS patients.The cDNAs for EPO have been cloned from human, mouse, canine, etc. The mature proteins from the various species are highly conserved, exhibiting greater than 80 % sequence identity at the amino acid level. Human EPO cDNA encodes a 193 amino acid residue precursor protein that is processed to yield a 165 amino acid residue mature protein. EPO contains one O-linked and three N-linked glycosylation sites. Glycosylation of EPO is required for EPO biological activities in vivo. EPO exhibits structural as well as amino sequence identity to the amino terminal 153 amino acid region of thrombopoietin.
Synonyms: Human EPO-alpha, EPO-alpha, EPO alpha, EPOalpha, h-EPO-alpha, rh-AEPO-alpha, recombinant human EPO-alpha, recombinant EPO-alpha, EPO.
Molecular Weight 21 kDa, observed 26-36 kDa in SDS-PAGE.
Pathways JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
Application Notes Mature human EPO, containing 166 amino acid residues, has a predicted molecular mass of approximately 21 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 26-36 kDa in SDS-PAGE.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/mL.
Buffer Lyophilized after extensive dialysis against PBS.
Storage -80 °C
Storage Comment Lyophilized recombinant human EPO remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhEPO should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.
Expiry Date 6 months
Supplier Images
SDS-PAGE (SDS) image for Erythropoietin (EPO) (AA 28-193) (Active) protein (ABIN987828) 5 μg of EPO-α, Human was resolved with SDS-PAGE under reducing (R) and non-reducing ...
 image for Erythropoietin (EPO) (AA 28-193) (Active) protein (ABIN987828) Erythropoietin (EPO) (AA 28-193) (Active) protein (Image 2)
Activity Assay (AcA) image for Erythropoietin (EPO) (AA 28-193) (Active) protein (ABIN987828) EPO-α, Human stimulates cell proliferation of TF-1 cells. The ED50 for this effect 0....
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