KERA Protein (AA 253-351) (GST tag)
(2 references)-
- Target See all KERA Proteins
- KERA (Keratocan (KERA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 253-351
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This KERA protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- KERA (Human) Recombinant Protein (Q01)
- Sequence
- GLPSRGFDVS SILDLQLSHN QLTKVPRISA HLQHLHLDHN KIKSVNVSVI CPSPSMLPAE RDSFSYGPHL RYLRLDGNEI KPPIPMALMT CFRLLQAVI
- Characteristics
- Human KERA partial ORF ( NP_008966, 253 a.a. - 351 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Keratocan (KERA) (AA 21-352) protein (His tag)
Crystallography grade KERA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Keratocan (KERA) (AA 21-351) protein (His tag)Crystallography grade KERA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Resistance of corneal RFUVA–cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases." in: Investigative ophthalmology & visual science, Vol. 54, Issue 2, pp. 1014-25, (2013) (PubMed).
: "Effects of ultraviolet-A and riboflavin on the interaction of collagen and proteoglycans during corneal cross-linking." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13011-22, (2011) (PubMed).
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: "Resistance of corneal RFUVA–cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases." in: Investigative ophthalmology & visual science, Vol. 54, Issue 2, pp. 1014-25, (2013) (PubMed).
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- Target
- KERA (Keratocan (KERA))
- Alternative Name
- KERA (KERA Products)
- Synonyms
- KERA Protein, si:dkeyp-38g8.3 Protein, zgc:136259 Protein, CNA2 Protein, SLRR2B Protein, keratocan Protein, KERA Protein, kera Protein, Kera Protein
- Background
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Full Gene Name: keratocan
Synonyms: CNA2,SLRR2B - Gene ID
- 11081
- NCBI Accession
- NM_007035
- Pathways
- Glycosaminoglycan Metabolic Process
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