MAP1LC3A Protein (AA 1-121) (GST tag)
(3 references)-
- Target See all MAP1LC3A Proteins
- MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-121
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This MAP1LC3A protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- MAP1LC3A (Human) Recombinant Protein (P01)
- Sequence
- MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE QEKDEDGFLY MVYASQETFG F
- Characteristics
- Human MAP1LC3A full-length ORF ( AAH15810.1, 1 a.a. - 121 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-120) protein (His-IF2DI Tag)
MAP1LC3A Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-102) protein (GST tag)MAP1LC3A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 2-120) protein (His tag)Crystallography grade MAP1LC3A Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-120) protein (His tag)Crystallography grade MAP1LC3A Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Prospective neoadjuvant analysis of PET imaging and mechanisms of resistance to Trastuzumab shows role of HIF1 and autophagy." in: British journal of cancer, Vol. 110, Issue 9, pp. 2209-16, (2014) (PubMed).
: "Immunohistochemical analysis of macroautophagy: recommendations and limitations." in: Autophagy, Vol. 9, Issue 3, pp. 386-402, (2013) (PubMed).
: "LC3 immunostaining pitfalls." in: Histopathology, Vol. 62, Issue 6, pp. 962-3, (2013) (PubMed).
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: "Prospective neoadjuvant analysis of PET imaging and mechanisms of resistance to Trastuzumab shows role of HIF1 and autophagy." in: British journal of cancer, Vol. 110, Issue 9, pp. 2209-16, (2014) (PubMed).
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- Target
- MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
- Alternative Name
- MAP1LC3A (MAP1LC3A Products)
- Synonyms
- ATG8E Protein, LC3 Protein, LC3A Protein, MAP1ALC3 Protein, MAP1BLC3 Protein, 1010001H21Rik Protein, 4922501H04Rik Protein, LC3a Protein, MGC69006 Protein, zgc:77094 Protein, MGC89867 Protein, MAP1LC3A Protein, map1lc3a Protein, microtubule associated protein 1 light chain 3 alpha Protein, microtubule-associated protein 1 light chain 3 alpha Protein, microtubule associated protein 1 light chain 3 alpha S homeolog Protein, MAP1LC3A Protein, Map1lc3a Protein, map1lc3a.S Protein, map1lc3a Protein
- Background
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Full Gene Name: microtubule-associated protein 1 light chain 3 alpha
Synonyms: LC3,LC3A,MAP1ALC3,MAP1BLC3 - Gene ID
- 84557
- Pathways
- Autophagy
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