MAP2K5 Protein (AA 1-448) (GST tag)

Catalog No. ABIN1310183

This Recombinant MAP2K5 protein is expressed in Wheat germ and has been mentioned in 2+ publications.

Quick Overview for MAP2K5 Protein (AA 1-448) (GST tag) (ABIN1310183)

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-448

Purification tag / Conjugate: This MAP2K5 protein is labelled with GST tag.

Purpose: MAP2K5 (Human) Recombinant Protein (P01)

Sequence: MLWLALGPFP AMENQVLVIR IKIPNSGAVD WTVHSGPQLL FRDVLDVIGQ VLPEATTTAF EYEDEDGDRI TVRSDEEMKA MLSYYYSTVM EQQVNGQLIE PLQIFPRACK PPGERNIHGL KVNTRAGPSQ HSSPAVSDSL PSNSLKKSSA ELKKILANGQ MNEQDIRYRD TLGHGNGGTV YKAYHVPSGK ILAVKVILLD ITLELQKQIM SELEILYKCD SSYIIGFYGA FFVENRISIC TEFMDGGSLD VYRKMPEHVL GRIAVAVVKG LTYLWSLKIL HRDVKPSNML VNTRGQVKLC DFGVSTQLVN SIAKTYVGTN AYMAPERISG EQYGIHSDVW SLGISFMELA LGRFPYPQIQ KNQGSLMPLQ LLQCIVDEDS PVLPVGESSE PFVHFITQCM RKQPKERPAP EELMGHPFIV QFNDGNAAVV SMWVCRALEE RRSQQGPP

Characteristics: Human MAP2K5 full-length ORF ( AAH08838, 1 a.a. - 448 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Takeda, Oberoi-Khanuja, Glatz, Schulenburg, Scholz, Carpy, Macek, Remenyi, Rajalingam: "Ubiquitin-dependent regulation of MEKK2/3-MEK5-ERK5 signaling module by XIAP and cIAP1." in: The EMBO journal, Vol. 33, Issue 16, pp. 1784-801, (2014) (PubMed).

García-Hoz, Sánchez-Fernández, Díaz-Meco, Moscat, Mayor, Ribas: "G alpha(q) acts as an adaptor protein in protein kinase C zeta (PKCzeta)-mediated ERK5 activation by G protein-coupled receptors (GPCR)." in: The Journal of biological chemistry, Vol. 285, Issue 18, pp. 13480-9, (2010) (PubMed).

Target Details

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Alternative Name: MAP2K5

Background: Full Gene Name: mitogen-activated protein kinase kinase 5
Synonyms: HsT17454,MAPKK5,MEK5,PRKMK5

Gene ID: 5607

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway