Midline 2 Protein (MID2) (AA 1-685) (GST tag)
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- Target See all Midline 2 (MID2) Proteins
- Midline 2 (MID2)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-685
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Midline 2 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- MID2 (Human) Recombinant Protein (P01)
- Sequence
- METLESELTC PICLELFEDP LLLPCAHSLC FSCAHRILVS SCSSGESIEP ITAFQCPTCR YVISLNHRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSES RRERTYRPTT AMSSERIACQ FCEQDPPRDA VKTCITCEVS YCDRCLRATH PNKKPFTSHR LVEPVPDTHL RGITCLDHEN EKVNMYCVSD DQLICALCKL VGRHRDHQVA SLNDRFEKLK QTLEMNLTNL VKRNSELENQ MAKLIQICQQ VEVNTAMHEA KLMEECDELV EIIQQRKQMI AVKIKETKVM KLRKLAQQVA NCRQCLERST VLINQAEHIL KENDQARFLQ SAKNIAERVA MATASSQVLI PDINFNDAFE NFALDFSREK KLLEGLDYLT APNPPSIREE LCTASHDTIT VHWISDDEFS ISSYELQYTI FTGQANFISL YNSVDSWMIV PNIKQNHYTV HGLQSGTRYI FIVKAINQAG SRNSEPTRLK TNSQPFKLDP KMTHKKLKIS NDGLQMEKDE SSLKKSHTPE RFSGTGCYGA AGNIFIDSGC HYWEVVMGSS TWYAIGIAYK SAPKNEWIGK NASSWVFSRC NSNFVVRHNN KEMLVDVPPH LKRLGVLLDY DNNMLSFYDP ANSLHLHTFD VTFILPVCPT FTIWNKSLMI LSGLPAPDFI DYPERQECNC RPQESPYVSG MKTCH
- Characteristics
- Human MID2 full-length ORF ( NP_438112.1, 1 a.a. - 685 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Midline 2 (MID2) (AA 371-460) protein (GST tag)
MID2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Midline 2 (MID2) (AA 1-735) protein (Strep Tag)Crystallography grade MID2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Midline 2 (MID2) (AA 1-705) protein (Strep Tag)Crystallography grade MID2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- Midline 2 (MID2)
- Alternative Name
- MID2 (MID2 Products)
- Synonyms
- MID2 Protein, fxy2 Protein, rnf60 Protein, trim1 Protein, FXY2 Protein, RNF60 Protein, TRIM1 Protein, Trim1 Protein, RGD1565674 Protein, midline 2 Protein, midline 2 L homeolog Protein, MID2 Protein, mid2 Protein, mid2.L Protein, Mid2 Protein
- Background
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Full Gene Name: midline 2
Synonyms: FXY2,MID1,RNF60,TRIM1 - Gene ID
- 11043
- NCBI Accession
- NM_052817
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