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Nemo-Like Kinase Protein (NLK) (AA 1-515) (GST tag)

Recombinant Nemo-Like Kinase protein expressed in Wheat germ.

Kinase Activity Assay (KAA) image for Nemo-Like Kinase (NLK) (AA 1-515) protein (GST tag) (ABIN1312656) (Nemo-Like Kinase Protein (NLK) (AA 1-515) (GST tag))

Image no. 2 for Nemo-Like Kinase (NLK) (AA 1-515) protein (GST tag) (ABIN1312656) (Nemo-Like Kinase Protein (NLK) (AA 1-515) (GST tag))

2 images

Catalog No. ABIN1312656

$475.39

Plus shipping costs $50.00, if applicable $20.00 dry ice

Shipping to: United States

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Quick Overview for Nemo-Like Kinase Protein (NLK) (AA 1-515) (GST tag) (ABIN1312656)

Target

See all Nemo-Like Kinase (NLK) Proteins

Nemo-Like Kinase (NLK)

Protein Type

Recombinant

Origin

Human

Source

Wheat germ

Application

Kinase Activity Assay (KAA)

Protein Characteristics

AA 1-515

Purification tag / Conjugate

This Nemo-Like Kinase protein is labelled with GST tag.

Purpose

NLK (Human) Recombinant Protein (P01)

Sequence

MAAYNGGTSA AATGHHHHHH HHLPHLPPPH LHHHHHPQHH LHPGSAAAVH PVQQHTSSAA AAAAAAAAAA AMLNPGQQQP YFPSPAPGQA PGPAAAAPAQ VQAAAAATVK AHHHQHSHHP QQQLDIEPDR PIGYGAFGVV WSVTDPRDGK RVALKKMPNV FQNLVSCKRV FRELKMLCFF KHDNVLSALD ILQPPHIDYF EEIYVVTELM QSDLHKIIVS PQPLSSDHVK VFLYQILRGL KYLHSAGILH RDIKPGNLLV NSNCVLKICD FGLARVEELD ESRHMTQEVV TQYYRAPEIL MGSRHYSNAI DIWSVGCIFA ELLGRRILFQ AQSPIQQLDL ITDLLGTPSL EAMRTACEGA KAHILRGPHK QPSLPVLYTL SSQATHEAVH LLCRMLVFDP SKRISAKDAL AHPYLDEGRL RYHTCMCKCC FSTSTGRVYT SDFEPVTNPK FDDTFEKNLS SVRQVKEIIH QFILEQQKGN RVPLCINPQS AAFKSFISST VAQPSEMPPS PLVWE

Characteristics

Human NLK full-length ORF ( AAH64663.1, 1 a.a. - 515 a.a.) recombinant protein with GST-tag at N-terminal.

Purification

in vitro wheat germ expression system
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Nemo-Like Kinase (NLK) (Active) protein (His-GST)
NLK Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 91 % as determined by reducing SDS-PAGE. Active

Nemo-Like Kinase (NLK) (Active) protein (GST tag)
NLK Origin: Human Host: Baculovirus infected Insect Cells Recombinant Active

Nemo-Like Kinase (NLK) (Active) protein (GST tag)
NLK Origin: Human Host: Insect Cells Recombinant 70 % - 90 % Func Active

Nemo-Like Kinase (NLK) (AA 1-527) protein (Strep Tag)
custom-made NLK Origin: Human Host: Cell-free protein synthesis (CFPS) Recombinant approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, WB, SDS

Nemo-Like Kinase (NLK) (AA 1-527) protein (Strep Tag)
custom-made NLK Origin: Mouse Host: Cell-free protein synthesis (CFPS) Recombinant approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, WB, SDS

Nemo-Like Kinase (NLK) protein (Myc-DYKDDDDK Tag)
NLK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD

Nemo-Like Kinase (NLK) (AA 1-527) protein (His tag)
NLK Origin: Rat Host: Yeast Recombinant > 90 % ELISA

Nemo-Like Kinase (NLK) protein
NLK Origin: Human Host: Baculovirus infected Insect Cells Recombinant Func, AcA, ScA

Application Notes

Suggested Applications*:elisa,kinase activity assay,antibody array,western blotting,affinity purification
*We do not guarantee this product working for suggested applications.
Optimal working dilution should be determined by the investigator.

Comment

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions

For Research Use only
Buffer

50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-80 °C

Storage Comment

Best use within three months from the date of receipt of this protein.
Target

Nemo-Like Kinase (NLK)

Alternative Name

NLK

Background

Full Gene Name: nemo-like kinase
Synonyms: DKFZp761G1211,FLJ21033

Gene ID

51701

Pathways

Ubiquitin Proteasome Pathway

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