NSF Protein (AA 1-744) (GST tag)
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- Target See all NSF Proteins
- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-744
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This NSF protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- NSF (Human) Recombinant Protein (P01)
- Sequence
- MAGRSMQAAR CPTDELSLTN CAVVNEKDFQ SGQHVIVRTS PNHRYTFTLK THPSVVPGSI AFSLPQRKWA GLSIGQEIEV SLYTFDKAKQ CIGTMTIEID FLQKKSIDSN PYDTDKMAAE FIQQFNNQAF SVGQQLVFSF NEKLFGLLVK DIEAMDPSIL KGEPATGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALLREEGASP LDFD
- Characteristics
- Human NSF full-length ORF ( AAH30613.1, 1 a.a. - 744 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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N-Ethylmaleimide-Sensitive Factor (NSF) (AA 1-744) protein (Strep Tag)
Crystallography grade NSF Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
N-Ethylmaleimide-Sensitive Factor (NSF) (AA 1-744) protein (Strep Tag)Crystallography grade NSF Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
N-Ethylmaleimide-Sensitive Factor (NSF) (AA 7-209), (partial) protein (His tag)NSF Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
N-Ethylmaleimide-Sensitive Factor (NSF) protein (Myc-DYKDDDDK Tag)NSF Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
- Alternative Name
- NSF (NSF Products)
- Synonyms
- SKD2 Protein, AI316878 Protein, AU020090 Protein, AU067812 Protein, NSF Protein, 18.m06598 Protein, nsf Protein, si:bz18k17.1 Protein, wu:fj33g11 Protein, N-ethylmaleimide sensitive factor Protein, T1J1.4 Protein, T1J1_4 Protein, NEM-sensitive fusion protein Protein, N-ethylmaleimide sensitive factor, vesicle fusing ATPase Protein, N-ethylmaleimide sensitive fusion protein Protein, N-ethylmaleimide-sensitive factor Protein, N-ethylmaleimide sensitive factor Protein, N-ethylmaleimide-sensitive fusion protein, putative Protein, N-ethylmaleimide sensitive factor L homeolog Protein, vesicle-fusing ATPase Protein, N-ethylmaleimide-sensitive factor a Protein, AAA-type ATPase family protein Protein, NSF Protein, Nsf Protein, nsf Protein, BBOV_II007200 Protein, TGME49_318510 Protein, nsf.L Protein, LOC100180685 Protein, LOC9310886 Protein, nsfa Protein
- Background
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Full Gene Name: N-ethylmaleimide-sensitive factor
Synonyms: SKD2 - Gene ID
- 4905
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