PEPCK Protein (AA 1-640) (GST tag)

Catalog No. ABIN1314318

This Recombinant PEPCK protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for PEPCK Protein (AA 1-640) (GST tag) (ABIN1314318)

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-640

Purification tag / Conjugate: This PEPCK protein is labelled with GST tag.

Purpose: PCK2 (Human) Recombinant Protein (P01)

Sequence: MAALYRPGLR LNWHGLSPLG WPSCRSIQTL RVLSGDLGQL PTGIRDFVEH SARLCQPEGI HICDGTEAEN TATLTLLEQQ GLIRKLPKYN NCWLARTDPK DVARVESKTV IVTPSQRDTV PLPPGGARGQ LGNWMSPADF QRAVDERFPG CMQGRTMYVL PFSMGPVGSP LSRIGVQLTD SAYVVASMRI MTRLGTPVLQ ALGDGDFVKC LHSVGQPLTG QGEPVSQWPC NPEKTLIGHV PDQREIISFG SGYGGNSLLG KKCFALRIAS RLARDEGWLA EHMLILGITS PAGKKRYVAA AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGR LRAINPENGF FGVAPGTSAT TNPNAMATIQ SNTIFTNVAE TSDGGVYWEG IDQPLPPGVT VTSWLGKPWK PGDKEPCAHP NSRFCAPARQ CPIMDPAWEA PEGVPIDAII FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS ESTAAAEHKG KIIMHDPFAM RPFFGYNFGH YLEHWLSMEG RKGAQLPRIF HVNWFRRDEA GHFLWPGFGE NARVLDWICR RLEGEDSARE TPIGLVPKEG ALDLSGLRAI DTTQLFSLPK DFWEQEVRDI RSYLTEQVNQ DLPKEVLAEL EALERRVHKM

Characteristics: Human PCK2 full-length ORF ( AAH01454, 1 a.a. - 640 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Kanno, Watanabe, Takahashi, Abe, Ohira: "Anti-phosphoenolpyruvate carboxykinase 2 antibody in patients with autoimmune hepatitis." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 44, Issue 9, pp. 1019-25, (2014) (PubMed).

Target Details

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Alternative Name: PCK2

Background: Full Gene Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Synonyms: PEPCK,PEPCK-M,PEPCK2

Gene ID: 5106

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis