PEPCK Protein (AA 33-640) (His-GST)

Catalog No. ABIN7197336

This Recombinant PEPCK protein is produced in Baculovirus infected Insect Cells.

Quick Overview for PEPCK Protein (AA 33-640) (His-GST) (ABIN7197336)

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 33-640

Purification tag / Conjugate: This PEPCK protein is labelled with His-GST.

Purpose: Recombinant Human PCK2 Protein (His & GST Tag)

Sequence: Leu33-Met640

Characteristics: A DNA sequence encoding the human PCK2 (NP_004554.3) (Leu33-Met640) was expressed with a C-terminal polyhistidine-tagged GST tag at the N-terminus (his-GST).

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 %glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Alternative Name: PCK2

Background: PEPCK,PEPCK-M,PEPCK2,PCK2 (Phosphoenolpyruvate Carboxykinase 2, Mitochondrial) is a Protein Coding gene. Diseases associated with PCK2 include Pepck 1 Deficiency and Phosphoenolpyruvate Carboxykinase Deficiency, Mitochondrial. Among its related pathways are Glycosaminoglycan metabolism and DNA Damage Response (only ATM dependent). Gene Ontology (GO) annotations related to this gene include GTP binding and purine nucleotide binding. An important paralog of this gene is PCK1.

Molecular Weight: 94.9 kDa

Gene ID: 5106

NCBI Accession: NP_004554

UniProt: Q16822

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis