Prohibitin Protein (PHB) (AA 1-272) (GST tag)
(1 reference)-
- Target See all Prohibitin (PHB) Proteins
- Prohibitin (PHB)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-272
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Prohibitin protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PHB (Human) Recombinant Protein (P01)
- Sequence
- MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVVFD RFRGVQDIVV GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA SQLPRIFTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAGDIAYQ LSRSRNITYL PAGQSVLLQL PQ
- Characteristics
- Human PHB full-length ORF ( AAH13401, 1 a.a. - 272 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Prohibitin (PHB) (AA 1-272) protein (His-IF2DI Tag)
PHB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Prohibitin (PHB) (AA 2-272) protein (His tag)Crystallography grade PHB Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Prohibitin (PHB) (AA 2-272) protein (His tag)Crystallography grade PHB Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Prohibitin (PHB) (AA 1-272), (full length) protein (His tag)PHB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Prohibitin (PHB) protein (Myc-DYKDDDDK Tag)PHB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Prohibitin interacts with phosphatidylinositol 3,4,5-triphosphate (PIP3) and modulates insulin signaling." in: Biochemical and biophysical research communications, Vol. 390, Issue 3, pp. 1023-8, (2009) (PubMed).
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: "Prohibitin interacts with phosphatidylinositol 3,4,5-triphosphate (PIP3) and modulates insulin signaling." in: Biochemical and biophysical research communications, Vol. 390, Issue 3, pp. 1023-8, (2009) (PubMed).
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- Target
- Prohibitin (PHB)
- Alternative Name
- PHB (PHB Products)
- Synonyms
- phb1 Protein, MGC75944 Protein, PHB Protein, Phb Protein, ACYPI000080 Protein, phb Protein, PHB1 Protein, Bap32 Protein, sr:nyz128 Protein, wu:fa18f07 Protein, wu:fb09f05 Protein, prohibitin1 Protein, xphb1 Protein, prohibitin Protein, prohibitin-2 Protein, prohibitin L homeolog Protein, PHB Protein, phb Protein, LOC484431 Protein, LOC5576054 Protein, Phb Protein, phb.L Protein
- Background
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Full Gene Name: prohibitin
Synonyms: PHB1 - Gene ID
- 5245
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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