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Prosaposin Protein (PSAP) (AA 18-524) (GST tag)

PSAP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA

Image no. 1 for Prosaposin (PSAP) (AA 18-524) protein (GST tag) (ABIN1316504) (Prosaposin Protein (PSAP) (AA 18-524) (GST tag))

1 image

(2 references)

Catalog No. ABIN1316504

Datasheet as PDF

Target See all Prosaposin (PSAP) Proteins

Prosaposin (PSAP)

Protein Type

Recombinant

Protein Characteristics

AA 18-524
Origin
10

6

1

1

1

1
Human
Source
12

3

2

2

1
Wheat germ

Purification tag / Conjugate

This Prosaposin protein is labelled with GST tag.

Application

Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose

PSAP (Human) Recombinant Protein (P01)

Sequence

PVLGLKECTR GSAVWCQNVK TASDCGAVKH CLQTVWNKPT VKSLPCDICK DVVTAAGDML KDNATEEEIL VYLEKTCDWL PKPNMSASCK EIVDSYLPVI LDIIKGEMSR PGEVCSALNL CESLQKHLAE LNHQKQLESN KIPELDMTEV VAPFMANIPL LLYPQDGPRS KPQPKDNGDV CQDCIQMVTD IQTAVRTNST FVQALVEHVK EECDRLGPGM ADICKNYISQ YSEIAIQMMM HMQPKEICAL VGFCDEVKEM PMQTLVPAKV ASKNVIPALE LVEPIKKHEV PAKSDVYCEV CEFLVKEVTK LIDNNKTEKE ILDAFDKMCS KLPKSLSEEC QEVVDTYGSS ILSILLEEVS PELVCSMLHL CSGTRLPALT VHVTQPKDGG FCEVCKKLVG YLDRNLEKNS TKQEILAALE KGCSFLPDPY QKQCDQFVAE YEPVLIEILV EVMDPSFVCL KIGACPSAHK PLLGTEKCIW GPSYWCQNTE TAAQCNAVEH CKRHVWN

Characteristics

Human PSAP full-length ORF ( AAH01503, 18 a.a. - 524 a.a.) recombinant protein with GST-tag at N-terminal.

Purification

in vitro wheat germ expression system
Prosaposin (PSAP) (AA 60-197) protein (His-IF2DI Tag)
PSAP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA

Prosaposin (PSAP) (AA 1-524) protein (GST tag)
PSAP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA

Prosaposin (PSAP) protein (His tag)
PSAP Origin: Human Host: HEK-293 Cells Recombinant > (79.8+19.7) % as determined by reducing SDS-PAGE..

Prosaposin (PSAP) (AA 195-273) protein (His tag)
Crystallography grade PSAP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys

Prosaposin (PSAP) (AA 311-390) protein (His tag)
Crystallography grade PSAP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys

Prosaposin (PSAP) (AA 195-274) protein (His tag)
Crystallography grade PSAP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys

Prosaposin (PSAP) (AA 405-486) protein (His tag)
Crystallography grade PSAP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys

Prosaposin (PSAP) (AA 60-142) protein (His tag)
Crystallography grade PSAP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys

Prosaposin (PSAP) (AA 194-275) protein (His tag)
Crystallography grade PSAP Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys

Prosaposin (PSAP) (AA 194-276) protein (His tag)
Crystallography grade PSAP Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys

Application Notes

Optimal working dilution should be determined by the investigator.

Comment

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions

For Research Use only
Buffer

50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-80 °C

Storage Comment

Best use within three months from the date of receipt of this protein.
Yamamoto-Tanaka, Motoyama, Miyai, Matsunaga, Matsuda, Tsuboi, Hibino: "Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation." in: The Journal of biological chemistry, Vol. 289, Issue 29, pp. 20026-38, (2014) (PubMed).

Wu, Sun, Zou, Xu, Liu, Wang, Yun, Gu: "Prosaposin, a regulator of estrogen receptor alpha, promotes breast cancer growth." in: Cancer science, Vol. 103, Issue 10, pp. 1820-5, (2012) (PubMed).
Target

Prosaposin (PSAP)

Alternative Name

PSAP (PSAP Products)

Synonyms

GLBA Protein, SAP1 Protein, AI037048 Protein, SGP-1 Protein, SGP1A Protein, cb759 Protein, wu:fa14a06 Protein, wu:fb36e02 Protein, wu:fb58g07 Protein, PSAP Protein, PSP-A Protein, SFTPA1B Protein, SP-A Protein, prosaposin Protein, surfactant protein A1 Protein, PSAP Protein, Psap Protein, psap Protein, LOC100011417 Protein, LOC100304726 Protein, LOC100526719 Protein, SFTPA1 Protein

Background

Full Gene Name: prosaposin
Synonyms: FLJ00245,GLBA,MGC110993,SAP1

Gene ID

5660

Pathways

Positive Regulation of Endopeptidase Activity

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