SPC25 Protein (AA 1-224) (GST tag)
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- Target See all SPC25 Proteins
- SPC25 (SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-224
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This SPC25 protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- SPC25 (Human) Recombinant Protein (P01)
- Sequence
- MVEDELALFD KSINEFWNKF KSTDTSCQMA GLRDTYKDSI KAFAEKLSVK LKEEERMVEM FLEYQNQISR QNKLIQEKKD NLLKLIAEVK GKKQELEVLT ANIQDLKEEY SRKKETISTA NKANAERLKR LQKSADLYKD RLGLEIRKIY GEKLQFIFTN IDPKNPESPF MFSLHLNEAR DYEVSDSAPH LEGLAEFQEN VRKTNNFSAF LANVRKAFTA TVYN
- Characteristics
- Human SPC25 full-length ORF ( NP_065726.1, 1 a.a. - 224 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25) (AA 1-224) protein (Strep Tag)
Crystallography grade SPC25 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25) (AA 1-226) protein (Strep Tag)Crystallography grade SPC25 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- SPC25 (SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25))
- Alternative Name
- SPC25 (SPC25 Products)
- Synonyms
- SPBC25 Protein, hSpc25 Protein, RGD1307811 Protein, Spbc25 Protein, spbc25 Protein, 2600017H08Rik Protein, 2610205L13Rik Protein, SPC25, NDC80 kinetochore complex component Protein, SPC25, NDC80 kinetochore complex component L homeolog Protein, SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) Protein, SPC25 Protein, Spc25 Protein, spc25.L Protein
- Background
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Full Gene Name: SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Synonyms: AD024,MGC22228,SPBC25 - Gene ID
- 57405
- NCBI Accession
- NM_020675
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