FIL1h Protein (AA 8-160) (His tag)
-
- Target See all FIL1h products
- FIL1h (Interleukin 1 eta (FIL1h))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 8-160
-
Origin
- Mouse
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This FIL1h protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Cross-Reactivity
- Mouse (Murine)
- Characteristics
- Mouse IL-36alpha (aa Arg8-His160) is untagged.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg purified protein (LAL test, Lonza).
-
Interleukin 1 eta (FIL1h) (Active) protein
FIL1h Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 1 eta (FIL1h) proteinFIL1h Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Interleukin 1 eta (FIL1h) (AA 31-183) protein (His tag)FIL1h Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 1 eta (FIL1h) (AA 5-157) protein (His tag)FIL1h Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 1 eta (FIL1h) (AA 1-157) protein (GST tag)FIL1h Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 1 eta (FIL1h) (AA 5-164) protein (His tag)Crystallography grade FIL1h Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Induces secretion of IL-6 in BMDCs at a concentration of 100ng - 1μg/ml.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitute with 100 μL sterile water.
- Concentration
- Lot specific
- Buffer
- Lyophilized. Contains PBS.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
-
- Target
- FIL1h (Interleukin 1 eta (FIL1h))
- Alternative Name
- IL-36 beta (FIL1h Products)
- Synonyms
- 2310043N20Rik Protein, Il36b Protein, FIL1 Protein, FIL1-(ETA) Protein, FIL1H Protein, FILI-(ETA) Protein, IL-1F8 Protein, IL-1H2 Protein, IL1-ETA Protein, IL1F8 Protein, IL1H2 Protein, Il1f8 Protein, interleukin 1 family, member 8 Protein, interleukin 36 beta Protein, interleukin 36, beta Protein, Il1f8 Protein, IL36B Protein, Il36b Protein
- Background
- IL-36alpha (IL-1F6), IL-36beta (IL-1F8) and IL-36gamma (IL-1F9) bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1 and are inhibited by IL-36Ra. The expression of IL-36 cytokines has been shown to occur at different sites including the lung and skin and can be derived from diverse cell types including keratinocytes, bronchial epithelium as well as macrophages, monocytes and different T cell subsets. IL-36 family members induce the production of proinflammatory cytokines, including IL-12, IL-1beta, IL-6, TNF-alpha and IL-23 in BMDC and CD4 T cells. Skin and dendritic cells are targets of the IL-36 interleukins leading to a Th1 response. These cytokines may represent potential targets for immune-mediated inflammatory conditions or, alternatively, could be used as adjuvants in vaccination. Recently a novel role for IL-36alpha in the pathogenesis of intestinal inflammation has been reported.
- Molecular Weight
- ~17kDa
- UniProt
- Q9JLA2
-
