NOTCH2 Protein (AA 26-494) (Fc Tag)

Catalog No. ABIN1344244

Recombinant NOTCH2 protein expressed in CHO Cells and has been mentioned in 1 publication.

Quick Overview for NOTCH2 Protein (AA 26-494) (Fc Tag) (ABIN1344244)

Target: NOTCH2 (Notch 2 (NOTCH2))

Protein Type: Recombinant

Origin: Mouse

Source: CHO Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 26-494

Purification tag / Conjugate: This NOTCH2 protein is labelled with Fc Tag.

Purpose: Notch2 (mouse):Fc (human) (rec.)

Specificity: Binds to mouse DLL1 and DLL4.

Cross-Reactivity: Mouse

Characteristics: The extracellular domain of mouse Notch2 (aa 26-494) (12 epidermal growth factor-like (EGF) repeats) is fused at the C-terminus to the Fc portion of human IgG1.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile water.

Concentration: 1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Fiorini, Merck, Wilson, Ferrero, Jiang, Koch, Auderset, Laurenti, Tacchini-Cottier, Pierres, Radtke, Luther, Macdonald: "Dynamic regulation of notch 1 and notch 2 surface expression during T cell development and activation revealed by novel monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 7212-22, (2009) (PubMed).

Target Details

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: Notch2

Background:

Neurogenic Locus Notch Homolog Protein 2, Motch B

Notch signaling pathway regulates many different cell fate decisions in both vertebrate and invertebrate species. There are 5 canonical Notch ligands in mammals: Jagged-1, Jagged-2, DLL1, DLL3 and DLL4. These can bind to the four Notch receptors Notch 1-4. It is important for pattern formation during development such as neurogenesis, angiogenesis or myogenesis and regulates T cell development and stem cell maintenance. Notch signaling is also involved in cellular processes through-out adulthood. Signaling via Notch occurs between neighbouring cells and both the receptor and its ligands are transmembrane proteins.

Molecular Weight: ~87kDa (SDS-PAGE)

UniProt: O35516

Pathways: Notch Signaling, Stem Cell Maintenance