NOTCH2 Protein (His tag)

Catalog No. ABIN7319906

Product Details

Target: NOTCH2 (Notch 2 (NOTCH2))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This NOTCH2 protein is labelled with His tag.

Purpose: Recombinant Human Notch2 (C-6His)

Sequence: Leu26-Gln530

Characteristics: Recombinant Human Neurogenic Locus Notch Homolog Protein 2 is produced by our Mammalian expression system and the target gene encoding Leu26-Gln530 is expressed with a 6His tag at the C-terminus.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: Notch2 (NOTCH2 Products)

Synonyms: AGS2 Protein, HJCYS Protein, hN2 Protein, AI853703 Protein, N2 Protein, notch6 Protein, sb:cb884 Protein, neurogenic locus notch homolog protein 2 Protein, notch 2 Protein, notch 3 Protein, LOC581186 Protein, NOTCH2 Protein, notch3 Protein, Notch2 Protein, notch2 Protein

Background:

Background: Notch-2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Human Notch-2 ECD (aa 26-530) shows 93 % , 93 %, 96 % and 96 % aa identity with the corresponding regions of mouse, rat, canine, and bovine Notch-2, respectively. Hajdu Cheney Syndrome (HCS) is a rare disease associated with mutations of NOTCH2 that lead to the translation of a truncated, presumably stable, NOTCH2 protein. NOTCH2 is down-regulated in colon cancer, and reduced expression is associated with a less differentiated, more aggressive phenotype, and reduced overall survival. NOTCH2 has also been shown to have pro-apoptotic and growth suppressive effects in thyroid carcinoma, and carcinoid tumors. NOTCH2 acts as an oncogene that promotes bladder cancer growth and metastasis through EMT, cell-cycle progression, and maintenance of stemness.

Synonym: AGS2, hN2, Notch homolog 2, Notch2, Notch-2, HJCYS

Molecular Weight: 54.9 kDa

UniProt: Q04721

Pathways: Notch Signaling, Stem Cell Maintenance