AKR1D1 Protein (AA 1-326) (GST tag)
(1 reference)-
- Target See all AKR1D1 Proteins
- AKR1D1 (Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-326
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This AKR1D1 protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)
- Purpose
- AKR1D1 (Human) Recombinant Protein (P01)
- Sequence
- MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY
- Characteristics
- Human AKR1D1 full-length ORF ( NP_005980.1, 1 a.a. - 326 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-326) protein (His tag)
AKR1D1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 227-326) protein (GST tag)AKR1D1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-326) protein (Strep Tag)Crystallography grade AKR1D1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-325) protein (Strep Tag)Crystallography grade AKR1D1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) protein (Myc-DYKDDDDK Tag)AKR1D1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "In vitro metabolism of a novel JNK inhibitor tanzisertib: interspecies differences in oxido-reduction and characterization of enzymes involved in metabolism." in: Xenobiotica; the fate of foreign compounds in biological systems, Vol. 45, Issue 6, pp. 465-80, (2015) (PubMed).
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: "In vitro metabolism of a novel JNK inhibitor tanzisertib: interspecies differences in oxido-reduction and characterization of enzymes involved in metabolism." in: Xenobiotica; the fate of foreign compounds in biological systems, Vol. 45, Issue 6, pp. 465-80, (2015) (PubMed).
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- Target
- AKR1D1 (Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))
- Alternative Name
- AKR1D1 (AKR1D1 Products)
- Synonyms
- 3o5bred Protein, CBAS2 Protein, SRD5B1 Protein, 3-oxo-5-beta-steroid 4-dehydrogenase-like Protein, aldo-keto reductase family 1 member D1 Protein, aldo-keto reductase family 1, member D1 Protein, aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) Protein, LOC491379 Protein, AKR1D1 Protein, Akr1d1 Protein
- Background
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Full Gene Name: aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase)
Synonyms: 3o5bred,SRD5B1 - Gene ID
- 6718
- NCBI Accession
- NM_005989
- Pathways
- Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process
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