Calpastatin Protein (CAST) (AA 1-686) (GST tag)
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- Target See all Calpastatin (CAST) Proteins
- Calpastatin (CAST)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-686
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Calpastatin protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- CAST (Human) Recombinant Protein (P01)
- Sequence
- MNPTETKAVK TEPEKKSQST KLSVVHEKKS QEGKPKEHTE PKSLPKQASD TGSNDAHNKK AVSRSAEQQP SEKSTEPKTK PQDMISAGGE SVAGITAISG KPGDKKKEKK SLTPAVPVES KPDKPSGKSG MDAALDDLID TLGGPEETEE ENTTYTGPEV SDPMSSTYIE ELGKREVTIP PKYRELLAKK EGITGPPADS SKPIGPDDAI DALSSDFTCG SPTAAGKKTE KEESTEVLKA QSAGTVRSAA PPQEKKRKVE KDTMSDQALE ALSASLGTRQ AEPELDLRSI KEVDEAKAKE EKLEKCGEDD ETIPSEYRLK PATDKDGKPL LPEPEEKPKP RSESELIDEL SEDFDRSECK EKPSKPTEKT EESKAAAPAP VSEAVCRTSM CSIQSAPPEP ATLKGTVPDD AVEALADSLG KKEADPEDGK PVMDKVKEKA KEEDREKLGE KEETIPPDYR LEEVKDKDGK PLLPKESKEQ LPPMSEDFLL DALSEDFSGP QNASSLKFED AKLAAAISEV VSQTPASTTQ AGAPPRDTSQ SDKDLDDALD KLSDSLGQRQ PDPDENKPME DKVKEKAKAE HRDKLGERDD TIPPEYRHLL DDNGQDKPVK PPTKKSEDSK KPADDQDPID ALSGDLDSCP STTETSQNTA KDKCKKAASS SKAPKNGGKA KDSAKTTEET SKPKDD
- Characteristics
- Human CAST full-length ORF ( NP_775083.1, 1 a.a. - 686 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Calpastatin (CAST) (AA 582-669) protein (GST tag)
CAST Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Calpastatin (CAST) (AA 1-708) protein (Strep Tag)Crystallography grade CAST Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Calpastatin (CAST) (AA 1-788) protein (Strep Tag)Crystallography grade CAST Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Calpastatin (CAST) (Transcript Variant 10) protein (Myc-DYKDDDDK Tag)CAST Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Calpastatin (CAST) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CAST Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Calpastatin (CAST) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)CAST Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- Calpastatin (CAST)
- Alternative Name
- CAST (CAST Products)
- Synonyms
- BS-17 Protein, calp3 Protein, CAST Protein, sb:cb966 Protein, zgc:194249 Protein, cast Protein, DKFZp469E0716 Protein, calpastatin Protein, calpastatin S homeolog Protein, Calpastatin Protein, CAST Protein, Cast Protein, cast.S Protein, cast Protein, VDBG_09182 Protein, ical Protein
- Background
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Full Gene Name: calpastatin
Synonyms: BS-17,MGC9402 - Gene ID
- 831
- NCBI Accession
- NM_173060
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