CH25H Protein (AA 1-272) (GST tag)
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- Target See all CH25H Proteins
- CH25H (Cholesterol 25-Hydroxylase (CH25H))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-272
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This CH25H protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- CH25H (Human) Recombinant Protein (P01)
- Sequence
- MSCHNCSDPQ VLCSSGQLFL QPLWDHLRSW EALLQSPFFP VIFSITTYVG FCLPFVVLDI LCSWVPALRR YKIHPDFSPS AQQLLPCLGQ TLYQHVMFVF PVTLLHWARS PALLPHEAPE LLLLLHHILF CLLLFDMEFF VWHLLHHKVP WLYRTFHKVH HQNSSSFALA TQYMSVWELF SLGFFDMMNV TLLGCHPLTT LTFHVVNIWL SVEDHSGYNF PWSTHRLVPF GWYGGVVHHD LHHSHFNCNF APYFTHWDKI LGTLRTASVP AR
- Characteristics
- Human CH25H full-length ORF ( NP_003947.1, 1 a.a. - 272 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Cholesterol 25-Hydroxylase (CH25H) (AA 206-272) protein (GST tag)
CH25H Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Cholesterol 25-Hydroxylase (CH25H) (AA 142-247) protein (GST tag)CH25H Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Cholesterol 25-Hydroxylase (CH25H) (AA 1-272) protein (Strep Tag)Crystallography grade CH25H Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Cholesterol 25-Hydroxylase (CH25H) (AA 1-298) protein (Strep Tag)Crystallography grade CH25H Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Cholesterol 25-Hydroxylase (CH25H) protein (Myc-DYKDDDDK Tag)CH25H Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- CH25H (Cholesterol 25-Hydroxylase (CH25H))
- Alternative Name
- CH25H (CH25H Products)
- Synonyms
- CH25H Protein, zgc:101688 Protein, MGC143045 Protein, C25H Protein, AI462618 Protein, m25OH Protein, cholesterol 25-hydroxylase Protein, cholesterol 25-hydroxylase L homeolog Protein, CH25H Protein, ch25h Protein, Ch25h Protein, ch25h.L Protein
- Background
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Full Gene Name: cholesterol 25-hydroxylase
Synonyms: C25H - Gene ID
- 9023
- NCBI Accession
- NM_003956
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