Dystroglycan Protein (AA 31-140) (GST tag)

Catalog No. ABIN1351078

Product Details

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Protein Type: Recombinant

Protein Characteristics: AA 31-140

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This Dystroglycan protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: DAG1 (Human) Recombinant Protein (Q01)

Sequence: WPSEPSEAVR DWENQLEASM HSVLSDLHEA VPTVVGIPDG TAVVGRSFRV TIPTDLIASS GDIIKVSAAG KEALPSWLHW DSQSHTLEGL PLDTDKGVHY ISVSATRLGA

Characteristics: Human DAG1 partial ORF ( AAH12740, 31 a.a. - 140 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Heng, Vollenhoven, Rombauts, Nie: "A High-Throughput Assay for the Detection of α-Dystroglycan N-Terminus in Human Uterine Fluid to Determine Uterine Receptivity." in: Journal of biomolecular screening, Vol. 21, Issue 4, pp. 408-13, (2016) (PubMed).

Hesse, Johansson, Mattsson, Bremell, Andreasson, Halim, Anckarsäter, Blennow, Anckarsäter, Zetterberg, Larson, Hagberg, Grahn: "The N-terminal domain of ?-dystroglycan, released as a 38 kDa protein, is increased in cerebrospinal fluid in patients with Lyme neuroborreliosis." in: Biochemical and biophysical research communications, Vol. 412, Issue 3, pp. 494-9, (2011) (PubMed).

Target Details

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Alternative Name: DAG1 (DAG1 Products)

Synonyms: LOC398500 Protein, dag1 Protein, MGC53537 Protein, 156DAG Protein, A3a Protein, AGRNR Protein, DAG Protein, MDDGC7 Protein, MDDGC9 Protein, DAG1 Protein, RAB7 Protein, dg Protein, a3a Protein, dag Protein, agrnr Protein, 156dag Protein, D9Wsu13e Protein, DG Protein, Dp427 Protein, Dp71 Protein, CG18250 Protein, CT41273 Protein, DmDG Protein, Dmel\\CG18250 Protein, atu Protein, dgn Protein, dys Protein, GB14967 Protein, APOJ Protein, CLI Protein, RATTRPM2B Protein, SGP-2 Protein, SGP2 Protein, SP-40 Protein, SP40 Protein, TRPM-2 Protein, TRPM2B Protein, Trpm2 Protein, Trpmb Protein, Ala-1 Protein, H9/25 Protein, Ly-27 Protein, Ly-6 Protein, Ly27 Protein, wu:fb83d06 Protein, wu:fi25f06 Protein, wu:fi37b08 Protein, zgc:109786 Protein, DystroGlycaN Protein, dystroglycan 1 L homeolog Protein, dystroglycan 1 Protein, dystroglycan 1 S homeolog Protein, Dystroglycan Protein, dystroglycan Protein, clusterin Protein, lymphocyte antigen 6 complex Protein, dgn-1 Protein, dag1.L Protein, dgn-2 Protein, dgn-3 Protein, DAG1 Protein, dag1.S Protein, Dag1 Protein, dag1 Protein, Dg Protein, LOC408826 Protein, Clu Protein, Ly6 Protein

Background: Full Gene Name: dystroglycan 1 (dystrophin-associated glycoprotein 1)
Synonyms: 156DAG,A3a,AGRNR,DAG

Gene ID: 1605

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus