GAA Protein (AA 1-952) (GST tag)
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- Target See all GAA Proteins
- GAA (Glucosidase, Alpha, Acid (GAA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-952
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This GAA protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- GAA (Human) Recombinant Protein (P01)
- Sequence
- MGVRHPPCSH RLLAVCALVS LATAALLGHI LLHDFLLVPR ELSGSSPVLE ETHPAHQQGA SRPGPRDAQA HPGRPRAVPT QCDVPPNSRF DCAPDKAITQ EQCEARGCCY IPAKQGLQGA QMGQPWCFFP PSYPSYKLEN LSSSEMGYTA TLTRTTPTFF PKDILTLRLD VMMETENRLH FTIKDPANRR YEVPLETPRV HSRAPSPLYS VEFSEEPFGV IVHRQLDGRV LLNTTVAPLF FADQFLQLST SLPSQYITGL AEHLSPLMLS TSWTRITLWN RDLAPTPGAN LYGSHPFYLA LEDGGSAHGV FLLNSNAMDV VLQPSPALSW RSTGGILDVY IFLGPEPKSV VQQYLDVVGY PFMPPYWGLG FHLCRWGYSS TAITRQVVEN MTRAHFPLDV QWNDLDYMDS RRDFTFNKDG FRDFPAMVQE LHQGGRRYMM IVDPAISSSG PAGSYRPYDE GLRRGVFITN ETGQPLIGKV WPGSTAFPDF TNPTALAWWE DMVAEFHDQV PFDGMWIDMN EPSNFIRGSE DGCPNNELEN PPYVPGVVGG TLQAATICAS SHQFLSTHYN LHNLYGLTEA IASHRALVKA RGTRPFVISR STFAGHGRYA GHWTGDVWSS WEQLASSVPE ILQFNLLGVP LVGADVCGFL GNTSEELCVR WTQLGAFYPF MRNHNSLLSL PQEPYSFSEP AQQAMRKALT LRYALLPHLY TLFHQAHVAG ETVARPLFLE FPKDSSTWTV DHQLLWGEAL LITPVLQAGK AEVTGYFPLG TWYDLQTVPI EALGSLPPPP AAPREPAIHS EGQWVTLPAP LDTINVHLRA GYIIPLQGPG LTTTESRQQP MALAVALTKG GEARGELFWD DGESLEVLER GAYTQVIFLA RNNTIVNELV RVTSEGAGLQ LQKVTVLGVA TAPQQVLSNG VPVSNFTYSP DTKVLDICVS LLMGEQFLVS WC
- Characteristics
- Human GAA full-length ORF ( P10253, 1 a.a. - 952 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Glucosidase, Alpha, Acid (GAA) (AA 851-952) protein (GST tag)
GAA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glucosidase, Alpha, Acid (GAA) (AA 123-952) protein (His tag)Crystallography grade GAA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Glucosidase, Alpha, Acid (GAA) (AA 204-952) protein (His tag)Crystallography grade GAA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Glucosidase, Alpha, Acid (GAA) (AA 70-952) protein (His tag)Crystallography grade GAA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Glucosidase, Alpha, Acid (GAA) (AA 70-953) protein (His tag)Crystallography grade GAA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- GAA (Glucosidase, Alpha, Acid (GAA))
- Alternative Name
- GAA (GAA Products)
- Synonyms
- LYAG Protein, E430018M07Rik Protein, glucosidase alpha, acid Protein, glucosidase, alpha, acid Protein, transmembrane and coiled-coil domain family 1 Protein, glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) Protein, GAA Protein, Gaa Protein, TMCC1 Protein, gaa Protein
- Background
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Full Gene Name: glucosidase, alpha, acid
Synonyms: LYAG - Gene ID
- 2548
- UniProt
- P10253
- Pathways
- Cellular Glucan Metabolic Process
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