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VEGFR-1/Flt-1 (D3) (glycosylated), (Monomer), (Soluble) (Active) Protein

Origin: Human Host: Insect Cells Recombinant > 90 % by SDS-PAGE. Visualized by silver stain Active
Catalog No. ABIN1589607
  • Target
    VEGFR-1/Flt-1 (D3)
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    glycosylated, Monomer, Soluble
    Origin
    Human
    Source
    Insect Cells
    Sequence
    SKLKDPELSL KGTQHIMQAG QTLHLQCRGE AAHKWSLPEM VSKESERLSI TKSACGRNGK QFCSTLTLNT AQANHTGFYS CKYLAVPTSK KKETESAIYI FISDTGRPFV EMYSEIPEII HMTEGRELVI PCRVTSPNIT VTLKKFPLDT LIPDGKRIIW DSRKGFIISN ATYKEIGLLT CEATVNGHLY KTNYLTHRQT NTIIDVQIST PRPVKLLRGH TLVLNCTATT PLNTRVQMTW SYPDEKNKRA SVRRRIDQSN SHANIFYSVL TIDKMQNKDK GLYTCRVRSG PSFKSVNTSV HIYDKAFITV KHRKQQVLET VAGKRSY
    Characteristics
    Length (AA): 327
    Chromosomal location: 13q12
    The activity of sVEGFR-1(D3) was determined by its ability to inhibit the VEGF-A-induced proliferation of HUVECs.
    Purity
    > 90 % by SDS-PAGE. Visualized by silver stain
  • Comment

    Soluble Receptors

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    The lyophilized sVEGFR-1(D3) is soluble in water and most aqueous buffers and should be reconstituted in PBS to a concentration not lower than 100 ng/mL.
    Buffer
    PBS
    Storage
    -20 °C/-80 °C
    Storage Comment
    Lyophilized samples are stable for greater than six months at -20 °C to -70 °C. Reconstituted sVEGFR-1(D3) should be stored in working aliquots at -70 °C.
    Expiry Date
    6 months
  • Target
    VEGFR-1/Flt-1 (D3)
    Background
    Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 domain D1-3 (sVEGFR-1(D3)) is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein contains only the first 3 extracellular domains, which contain all the information necessary for binding of VEGF. The receptor monomers have a mass of approximately 45 kDa containing 352 amino acid residues. Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor.
    Synonyms: soluble vascular endothelial growth factor receptor-1, soluble FLT1, soluble VEGFR-1
    Molecular Weight
    45.0 kDa
    NCBI Accession
    NM_001159920, NP_001153392
    UniProt
    P17948
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