PDGF-BB Homodimer (Homodimer) (Active) Protein

Catalog No. ABIN1589629

Recombinant protein expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for PDGF-BB Homodimer (Homodimer) (Active) Protein (ABIN1589629)

Target: PDGF-BB Homodimer

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE

Product Details

Protein Characteristics: Homodimer

Purpose: PDGF-BB

Sequence: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

Specificity: Chromosomal location:22q13.1

Characteristics: Length (aa):109

Endotoxin Level: < 0.1 ng per ug of PDGF-BB

Application Details

Application Notes: The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. The lyophilized PDGF-BB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1 % ) is recommended.

Buffer: 50 mM acetic acid

Handling Advice: Centrifuge vial prior to opening.

Storage: RT,-20 °C,-80 °C

Storage Comment: The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-BB is best stored at -20°C to -70°C.

References

Rezzola, Dal Monte, Belleri, Bugatti, Chiodelli, Corsini, Cammalleri, Cancarini, Morbidelli, Oreste, Bagnoli, Semeraro, Presta: "Therapeutic Potential of Anti-Angiogenic Multitarget N,O-Sulfated E. Coli K5 Polysaccharide in Diabetic Retinopathy." in: Diabetes, Vol. 64, Issue 7, pp. 2581-92, (2015) (PubMed).

Target Details

Target: PDGF-BB Homodimer

Alternative Name: PDGF-BB

Background: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, INN=Becaplermin

Molecular Weight: 24.3 kDa

Gene ID: 5155

NCBI Accession: NM_002608, NP_002599

UniProt: P01127