LYVE1 Protein (Soluble) (His tag)

Catalog No. ABIN1589750

Product Details

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Protein Type: Recombinant

Protein Characteristics: Soluble

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This LYVE1 protein is labelled with His tag.

Sequence: ADLVQDLSIS TCRIMGVALV GRNKNPQMNF TEANEACKML GLTLASRDQV ESAQKSGFET CSYGWVGEQF SVIPRIFSNP RCGKNGKGVL IWNAPSSQKF KAYCHNSSDT WVNSCIPEIV TTFYPVLDTQ TPATEFSVSS SAYLASSPDS TTPVSATTRA PPLTSMARKT KKICITEVYT EPITMATETE AFVASGAAFK NEAAGHHHHH H

Characteristics: Length (AA): 211
Chromosomal location: 7, 7F2

Purity: > 95 % by SDS-PAGE. Visualized by silver stain

Application Details

Application Notes: Not tested so far!

Comment:

Soluble Receptors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized sLYVE-1 is soluble in water and most aqueous buffers. The lyophilized sLYVE-1 should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.

Buffer: PBS

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C/-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20 °C to -70 °C. Reconstituted sLYVE-1 should be stored in working aliquots at -20 °C.

Expiry Date: 6 months

Target Details

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Alternative Name: Lyve-1 (LYVE1 Products)

Synonyms: LYVE1 Protein, XLKD1 Protein, CRSBP-1 Protein, HAR Protein, LYVE-1 Protein, 1200012G08Rik Protein, Crsbp-1 Protein, Lyve-1 Protein, Xlkd1 Protein, lymphatic vessel endothelial hyaluronic receptor 1a Protein, lymphatic vessel endothelial hyaluronan receptor 1 Protein, lyve1a Protein, LYVE1 Protein, Lyve1 Protein

Background: A DNA sequence encoding the extracellular domain of mouse LYVE-1 (Met1 – Gly228) was fused to a C-terminal His-tag (6xHis) and expressed in insect cells. Based on N-terminal sequence analysis, the primary structure of recombinant mature sLYVE-1 starts at Ala24. sLYVE-1 has a calculated monomeric molecular mass of about 25 kDa but as a result of glycosylation, migrates at approximately 35 - 45 kDa under reducing conditions in SDS-PAGE. LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Synonyms: Lyve1, Xlkd1, Lyve-1, Crsbp-1, 1200012G08Rik

Molecular Weight: 35.0 - 45.0 kDa

NCBI Accession: NP_444477, NM_053247

UniProt: Q8BHC0

Pathways: Glycosaminoglycan Metabolic Process