TEK Protein (Soluble) (His tag)
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- Target See all TEK Proteins
- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
- Protein Type
- Recombinant
- Protein Characteristics
- Soluble
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This TEK protein is labelled with His tag.
- Purpose
- TIE-2, soluble
- Sequence
- AMDLILINSL PLVSDAETLT CIASGWHPHE PITIGRDFEA LMNQHQDPLE VTQDVTREWA KKVVWKREKA SKINGAYCEG RVRGQAIRIR TMKMRQQASF LPATLTMTVD RGDNVNISFK KVLIKEEDAV IYKNGSIHSV PRHEVPDILE VHLPHAQPQD AGVYSARYIG GNLFTSAFTR LIVRRCEAQK WGPDCRPCTT CKNNGVCHED TGECICPPGF MGRTCEKACE PHTFGRTCKE RCSGPEGCKS YVFCPDPYGC SCATGWRGLQ CNEACPSGYY GPDCKLRCHC TNEEICDRFQ GCLCSQGWQG LQCKEGRPRM TPQIEDLPDH IEVNSGKFNP ICKASGWPLP TSEEMTLVKP DGTVLQPNDF NYDRFSVAIF TVNRVLPPDS GVWVCSVNTV AGMVEKPFNI SVKVLPEPLH APNVIDTGHN FIINISSEPY FGDGPIKSKK LFYKPVNQAW KYIEVTNEIF TLNYLEPRTD YELCVQLARP EGGEGHPGPV RRFTTASIGL PPPRGLSLLP KSQTALNLTW QPIFTNSEDE FYVEVERRSQ TTSDQQNIKV PGNLTSVLLS NLVPREQYTV RARVNTKAQG EWSEELRAWT LSDILPPQEN IKISNITDST AMVSWTIVDG YSISSIIIRY KVQGKNEDQH IDVKIKNATV TQYQLKGEPE TTYHVDIFAE NNIGSSNPAF SHELRTLPHS PASATRHHHH HH
- Specificity
- Chromosomal location:4 C5, 4 43.34 cM
- Characteristics
- Length (aa):712
- Purity
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product TEK Protein
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TEK Tyrosine Kinase, Endothelial (TEK) (AA 23-748) (Active) protein (Fc Tag)
TEK Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
TEK Tyrosine Kinase, Endothelial (TEK) (Active) protein (His tag)TEK Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
TEK Tyrosine Kinase, Endothelial (TEK) (Active) protein (Fc Tag)TEK Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
TEK Tyrosine Kinase, Endothelial (TEK) (AA 770-1122) (Active) protein (GST tag,His tag)TEK Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 91 % as determined by SDS-PAGE Active
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- Comment
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Soluble Receptors
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge vial prior to opening. The lyophilized sTIE-2-His is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.
- Buffer
- PBS
- Storage
- -20 °C,-80 °C
- Storage Comment
- Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sTIE-2-His should be stored in working aliquots at -20°C.
- Expiry Date
- 6 months
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- Target
- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
- Alternative Name
- TIE-2 (TEK Products)
- Background
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Recombinant mouse soluble TIE-2 was fused with a 6x His-tag at the C-terminus. The soluble receptor protein consists of the full extracellular domain (Ala23-Ala737). Mouse sTIE-2 monomer has a calculated molecular mass of approximately 79,86 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 95 kDa protein in SDS-PAGE under reducing conditions. TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Synonyms: Angiopoietin-1 receptor, Endothelial tyrosine kinase, HYK, STK1, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, CD202b, Tek, Hyk, Tie2, AA517024 - Gene ID
- 21687
- NCBI Accession
- NM_013690, NP_038718
- UniProt
- Q02858
- Pathways
- RTK Signaling, Growth Factor Binding
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