TEK Protein (His tag)

Catalog No. ABIN7320055

This Recombinant TEK protein is expressed in HEK-293 Cells.

Quick Overview for TEK Protein (His tag) (ABIN7320055)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This TEK protein is labelled with His tag.

Purpose: Recombinant Mouse Tie2/CD202b Protein (His Tag)(Active)

Sequence: Met1-Lys744

Characteristics: A DNA sequence encoding the mouse TEK (Q02858) (Met1-Lys744) was expressed with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized mouse TEK-His at 10 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 6.25-200 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: Tie2/CD202b

Background:

Background: TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Synonym: AA517024,Cd202b,Hyk,STK1,Tie-2,Tie2

Molecular Weight: 82.4 kDa

UniProt: Q02858

Pathways: RTK Signaling, Growth Factor Binding