Glycerol kinase (glpK) (AA 1-492) protein (His tag)

Catalog No. ABIN1593902

Product Details

Target: Glycerol kinase (glpK)

Protein Type: Recombinant

Protein Characteristics: AA 1-492

Origin: Aquifex aeolicus

Source: Yeast

Purification tag / Conjugate: His tag

Application: ELISA

Sequence: MSVVISIDVG TTRVKVIAFS KNGKIVAISD REVSQIYPEP GWVEQDPLEL WEAVRKSLSE VIQQVGLKEI NSIGITNQRE TVILWDKETG RPVYNAILWQ DLRTEDICRK LSEYSEYIKE NTGLLLHPYF SASKVNWIIE NVNGVKKDIE RGKVIFGTVD TWILWNLTGG KVHKTEPSNA SRTLLFNIKR LEYDDELLKI FRIPKNILPE VNESSSLFGY TDKSITGVEI PITGILGDQQ ASLFAHGIRE LEEVKNTYGT GLFLMLLTGS KPIIPERLLG TVAWVINGKV NYAIEGSVLT GGACIKWLMD RLKLIKQAAD TEYLAKSISS NEGVYFVPAF SGLGAPYWDA SARGIIIGIT GRTRIEHIAR AALEAIAYQT RDVIEEMEKE TGVKIKILKA DGGASQNNFL MQFQADILGI PVERPRHVEL TALGAAGIAG IYSGMWKSKE EFIEDTREVE LTFSPEMKKE ERELYYSKWK DAVKRAMKWS ML

Specificity: Aquifex aeolicus (strain VF5)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: Glycerol kinase (glpK)

Background: Recommended name: Glycerol kinase.
EC= 2.7.1.30.
Alternative name(s): ATP:glycerol 3-phosphotransferase Glycerokinase.
Short name= GK

UniProt: O66746