ARHGEF18 Protein (AA 159-551, partial) (GST tag)

Catalog No. ABIN7479310

Product Details

Target: ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))

Protein Type: Recombinant

Protein Characteristics: AA 159-551, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ARHGEF18 protein is labelled with GST tag.

Application: ELISA

Sequence: MTVSQKGGPQ PTPSPAGPGT QLGPITGEMD EADSAFLKFK QTADDSLSLT SPNTESIFVE DPYTASLRSE IESDGHEFEA ESWSLAVDAA YAKKQKREVV KRQDVLYELM QTEVHHVRTL KIMLKVYSRA LQEELQFSSK AIGRLFPCAD DLLETHSHFL ARLKERRQES LEEGSDRNYV IQKIGDLLVQ QFSGENGERM KEKYGVFCSG HNEAVSHYKL LLQQNKKFQN LIKKIGNFSI VRRLGVQECI LLVTQRITKY PVLVERIIQN TEAGTEDYED LTQALNLIKD IISQVDAKVS ECEKGQRLRE IAGKMDLKSS SKLKNGLTFR KEDMLQRQLH LEGMLCWKTT SGRLKDILAI LLTDVLLLLQ EKDQKYVFAS VDSKPPVISL QKL

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 95 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Target Details

Target: ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))

Alternative Name: Rho guanine nucleotide exchange factor 18 protein (ARHGEF18 Products)

Synonyms: arhgef18 Protein, wu:fc21e04 Protein, wu:fj05f11 Protein, zgc:85686 Protein, P114-RhoGEF Protein, AI467246 Protein, D030053O22Rik Protein, rho/rac guanine nucleotide exchange factor (GEF) 18a Protein, Rho/Rac guanine nucleotide exchange factor (GEF) 18 Protein, Rho/Rac guanine nucleotide exchange factor 18 Protein, rho guanine nucleotide exchange factor 18 Protein, rho/rac guanine nucleotide exchange factor (GEF) 18 Protein, arhgef18a Protein, ARHGEF18 Protein, LOC100027283 Protein, Arhgef18 Protein

Background: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production.

Molecular Weight: 71.9 kD

UniProt: Q6ZSZ5

Pathways: Neurotrophin Signaling Pathway